|
Status |
Public on May 22, 2012 |
Title |
Stanford_ChipSeq_K562_UBF_(sc-13125)_IgG-mus |
Sample type |
SRA |
|
|
Source name |
K562
|
Organism |
Homo sapiens |
Characteristics |
lab: Stanford lab description: Snyder - Stanford University datatype: ChipSeq datatype description: Chromatin IP Sequencing cell: K562 cell organism: human cell description: leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC cell karyotype: cancer cell lineage: mesoderm cell sex: F treatment: None treatment description: No special treatment or protocol applies antibody: UBF_(sc-13125) antibody antibodydescription: UBF (F-9) is a mouse monoclonal antibody raised against amino acids 1-220 of UBF of human origin. Antibody Target: UBF antibody targetdescription: Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 (PubMed 3413483)). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes antibody vendorname: Santa Cruz Biotech antibody vendorid: sc-13125 control: IgG-mus control description: Input signal from Normal Mouse IgG ChIP-seq. control: IgG-mus control description: Input signal from Normal Mouse IgG ChIP-seq. controlid: wgEncodeEH000726 replicate: 1
|
Biomaterial provider |
ATCC
|
Treatment protocol |
None
|
Growth protocol |
K562_protocol.pdf
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
|
|
Submission date |
May 22, 2012 |
Last update date |
May 15, 2019 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL9052 |
Series (2) |
GSE31477 |
ENCODE Transcription Factor Binding Sites by ChIP-seq from Stanford/Yale/USC/Harvard |
GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
Relations |
SRA |
SRX150418 |
BioSample |
SAMN01000879 |
Named Annotation |
GSM935338_hg19_wgEncodeSydhTfbsK562Ubfsc13125IggmusSig.bigWig |