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Sample GSM935141 Query DataSets for GSM935141
Status Public on Aug 27, 2012
Title genomic DNA from sample F1 follow-up (Bisulfite-Seq)
Sample type SRA
Source name F1
Organism Homo sapiens
Characteristics sample id: F1
tissue: whole blood
Extracted molecule genomic DNA
Extraction protocol genomic DNA was extracted from dried blood spot samples using the GenSolve kit as per manufacturers instructures. DNA was extracted from other samples using the Qiagen DNeasy kit.
Bisulfite-seq libraries were prepared
Library strategy Bisulfite-Seq
Library source genomic
Library selection RANDOM
Instrument model Illumina Genome Analyzer IIx
Description normal blood sample
Data processing Standard Illumina software was used for processing and basecalling
Read pairs were aligned to the GRCh37 genome using BWA 0.5.9
Read pairs were filtered (quality score >= 10) and length-normalized within each group (DBS and followup)
Read pairs were counted in 500bp windows centered on the probes from the Illumina HumanMethylation450K array
Genome_build: GRCh37/hg19
Supplementary_files_format_and_content: Tab-delimited files countaining read counts
Genome Build:
Followup-F1.counts.txt: GRCh37/hg19
Submission date May 22, 2012
Last update date May 15, 2019
Contact name Thomas A Down
Organization name University of Cambridge
Department WT/CRUK Gurdon Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
Platform ID GPL10999
Series (2)
GSE38127 Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans (Bisulfite-Seq)
GSE38128 Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans
SRA SRX148968
BioSample SAMN00997693

Supplementary file Size Download File type/resource
GSM935141_Followup-F1.counts.txt.gz 2.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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