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Sample GSM934569 Query DataSets for GSM934569
Status Public on Dec 07, 2012
Title d11.5 placenta leptin 25
Sample type RNA
 
Source name d11.5 placenta, mom 126
Organism Mus musculus
Characteristics treatment: 50% food restriction w/ leptin replacement
tissue: placenta
maternal treatment group: leptin
pregnancy stage: d11.5
genetic background: Swiss Webster
Treatment protocol Mice were mated, and on pregnancy day 1.5, randomized to (1) ad libitum fed (control); (2) restricted to 50% of previous food consumption (restricted); (3) restricted to 50% and injected once daily with 1 mg/kg recombinant mouse leptin. Mice were sacrificed on d11.5 and placentas were collected.
Extracted molecule total RNA
Extraction protocol Placentas were halved and frozen in Trireagent (Sigma). After phase separation, the aqueous phase was applied to RNEasy columns (Qiagen) according to manufacturer's protocol. Total RNA from two placentas per mother were pooled.
Label Cy3
Label protocol First and second strand cDNA was prepared from the total RNA samples. cRNA target was prepared from the DNA template and verified on the Bioanalyzer.
 
Hybridization protocol Purified cRNA (1 µg) was fragmented to uniform size and applied to Agilent 4x44K Whole Mouse Genome microarrays (Design ID 026655, Agilent Technologies, Santa Clara, CA) in hybridization buffer. Arrays were hybridized at 65° C for 17 h on a rotating incubator and washed at 37° C for 1 min.
Scan protocol Rinsed and dried arrays were scanned with an Agilent G2565 Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 5 µm resolution.
Description pooled from two placentas
Data processing Agilent Feature Extraction software was used to process the scanned images from arrays (gridding and feature intensity extraction) and the data generated for each probe on the array was analyzed with GeneSpring GX v7.3.1 software (Agilent Technologies, Santa Clara, CA).
 
Submission date May 21, 2012
Last update date Dec 07, 2012
Contact name Laura Clamon Schulz
E-mail(s) SchulzL@missouri.edu
Organization name University of Missouri
Department Obstetrics, Gynecology and Women's Health
Street address 1 Hospital Dr.
City Columbia
State/province MO
ZIP/Postal code 65212
Country USA
 
Platform ID GPL11202
Series (1)
GSE38095 Leptin and the Placental Response to Maternal Food Restriction during Early Pregnancy

Data table header descriptions
ID_REF
VALUE Normalized values are normalized to 75th percentile intensity
Flags
gProcessedSignal

Data table
ID_REF VALUE Flags gProcessedSignal
A_55_P2055107 1.10 P 1529.22
A_55_P2125947 3.03 P 4224.11
A_55_P1970810 4.90 P 6831.60
A_55_P2152592 5.26 P 7332.68
A_51_P346165 4.35 P 6061.70
A_52_P572447 3.28 P 4568.05
A_52_P24986 3.78 P 5271.80
A_55_P1968433 2.70 P 3757.76
A_52_P625277 0.35 M 491.99
A_55_P2168073 0.01 A 17.16
A_55_P2100241 14.19 P 19775.62
A_51_P443293 2.72 P 3792.83
A_52_P24951 0.55 P 765.83
A_66_P122837 0.66 P 924.77
A_51_P481768 0.00 A 4.74
A_51_P143200 0.01 A 14.83
A_55_P2034928 0.09 P 123.13
A_51_P512820 1.86 P 2594.48
A_66_P128918 0.62 P 864.78
A_52_P259558 0.16 P 225.25

Total number of rows: 39429

Table truncated, full table size 1050 Kbytes.




Supplementary file Size Download File type/resource
GSM934569_125-Leptin.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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