NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM932551 Query DataSets for GSM932551
Status Public on May 18, 2012
Title KRAS-mutant case 7 (non tumor)
Sample type RNA
 
Source name nonneoplastic colon mucosa
Organism Homo sapiens
Characteristics genotype/variation: KRAS-mutant
Treatment protocol fresh frozen
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol Total RNA of normal and CRC tissue was isolated using the mirVana miRNA Isolation Kit (Ambion/ Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instruction. To digest DNA, the samples were treated with DNAse of the Turbo DNA-free kit (Ambion). RNA quality was assessed in a 1.5% agarose gel and concentrations of the samples were measured in a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, USA). RNAs were stored at -80°C.
Label biotin
Label protocol according to the manufacturer`s recommendations
 
Hybridization protocol according to the manufacturer`s recommendations
Scan protocol according to the manufacturer`s recommendations
Description KRAS-mutant colon cancer case # 7 (non tumor)
Data processing Data Mining Tool 3.0 (Affymetrix) and GeneSpring software package 7.2 (Silicon Genetics, Redwood City, CA)
 
Submission date May 17, 2012
Last update date May 18, 2012
Contact name Christoph Röcken
E-mail(s) christoph.roecken@uk-sh.de
Phone +49(0)4315973401
Fax +49(0)4315973462
Organization name Christian-Albrechts-University Kiel
Department Pathology
Street address Arnold-Heller-Str. 3/14
City Kiel
ZIP/Postal code D-24105
Country Germany
 
Platform ID GPL11532
Series (1)
GSE38026 KRAS-genotype dependent gene expression pattern in colorectal cancer

Data table header descriptions
ID_REF
VALUE log2 RMA

Data table
ID_REF VALUE
7896736 4.26
7896738 2.13
7896740 1.91
7896742 6.01
7896744 2.90
7896746 7.39
7896748 4.35
7896750 2.61
7896752 7.91
7896754 5.37
7896756 2.60
7896759 4.46
7896761 3.87
7896779 4.74
7896798 4.35
7896817 4.42
7896822 6.08
7896859 3.04
7896861 2.64
7896863 4.08

Total number of rows: 33257

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM932551_A548_31_N_18290_03.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap