|
Status |
Public on May 18, 2012 |
Title |
KRAS-mutant case 5 (non tumor) |
Sample type |
RNA |
|
|
Source name |
nonneoplastic colon mucosa
|
Organism |
Homo sapiens |
Characteristics |
genotype/variation: KRAS-mutant
|
Treatment protocol |
fresh frozen
|
Growth protocol |
not applicable
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA of normal and CRC tissue was isolated using the mirVana miRNA Isolation Kit (Ambion/ Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instruction. To digest DNA, the samples were treated with DNAse of the Turbo DNA-free kit (Ambion). RNA quality was assessed in a 1.5% agarose gel and concentrations of the samples were measured in a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, USA). RNAs were stored at -80°C.
|
Label |
biotin
|
Label protocol |
according to the manufacturer`s recommendations
|
|
|
Hybridization protocol |
according to the manufacturer`s recommendations
|
Scan protocol |
according to the manufacturer`s recommendations
|
Description |
KRAS-mutant colon cancer case # 5 (non tumor)
|
Data processing |
Data Mining Tool 3.0 (Affymetrix) and GeneSpring software package 7.2 (Silicon Genetics, Redwood City, CA)
|
|
|
Submission date |
May 17, 2012 |
Last update date |
May 18, 2012 |
Contact name |
Christoph Röcken |
E-mail(s) |
christoph.roecken@uk-sh.de
|
Phone |
+49(0)4315973401
|
Fax |
+49(0)4315973462
|
Organization name |
Christian-Albrechts-University Kiel
|
Department |
Pathology
|
Street address |
Arnold-Heller-Str. 3/14
|
City |
Kiel |
ZIP/Postal code |
D-24105 |
Country |
Germany |
|
|
Platform ID |
GPL11532 |
Series (1) |
GSE38026 |
KRAS-genotype dependent gene expression pattern in colorectal cancer |
|