|
| Status |
Public on Nov 01, 2013 |
| Title |
Normal 20 |
| Sample type |
genomic |
| |
|
| Source name |
Human Breast
|
| Organism |
Homo sapiens |
| Characteristics |
race: African-American
age: 45
tissue type: Non-tumor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from fresh frozen primary breast tumours following the Qiagen DNeasy Blood and Tissue Kit protocol. Briefly, tissues were pre-treated with Proteinase K at 55C over-night, then lysed, bound to the column, washed, and eluted. DNA was quantified using the nanodrop spectrophotomer.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Infinium HD Methylation Assay protocol
|
| Scan protocol |
Arrays were imaged using HiScan Reader using standard recommended Illumina scanner setting.
|
| Data processing |
GenomeStudio software v2010.3, Methylation module v1.8.5
|
| |
|
| Submission date |
May 03, 2012 |
| Last update date |
Nov 01, 2013 |
| Contact name |
Atsushi Terunuma |
| Organization name |
National Cancer Institute
|
| Department |
Dermatology Branch
|
| Street address |
37 Convent Drive, 37/3050
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE37754 |
Molecular Profiles of Human Breast Cancer and Their Association with Tumor Subtypes and Disease Prognosis (Illumina) |
| GSE39004 |
Molecular Profiles of Human Breast Cancer and Their Association with Tumor Subtypes and Disease Prognosis |
|
