|
Status |
Public on Nov 01, 2013 |
Title |
Tumor 55 |
Sample type |
genomic |
|
|
Source name |
Human Breast Tumor
|
Organism |
Homo sapiens |
Characteristics |
race: European-American age: 89 tissue type: Tumor estrogen receptor status: Positive triple negative or basal-like subtype: No Stage: IIIA tumor grade: 2 node status: N2 survival (months): 13 survival event: Dead death due to cancer: Yes neoadjuvant therapy: Yes hormone-therapy: Yes chemotherapy: No
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated from fresh frozen primary breast tumours following the Qiagen DNeasy Blood and Tissue Kit protocol. Briefly, tissues were pre-treated with Proteinase K at 55C over-night, then lysed, bound to the column, washed, and eluted. DNA was quantified using the nanodrop spectrophotomer.
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Standard Infinium HD Methylation Assay protocol
|
Scan protocol |
Arrays were imaged using HiScan Reader using standard recommended Illumina scanner setting.
|
Data processing |
GenomeStudio software v2010.3, Methylation module v1.8.5
|
|
|
Submission date |
May 03, 2012 |
Last update date |
Nov 01, 2013 |
Contact name |
Atsushi Terunuma |
Organization name |
National Cancer Institute
|
Department |
Dermatology Branch
|
Street address |
37 Convent Drive, 37/3050
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE37754 |
Molecular Profiles of Human Breast Cancer and Their Association with Tumor Subtypes and Disease Prognosis (Illumina) |
GSE39004 |
Molecular Profiles of Human Breast Cancer and Their Association with Tumor Subtypes and Disease Prognosis |
|