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Sample GSM925236 Query DataSets for GSM925236
Status Public on May 02, 2012
Title Prolonged_wakefulness_volunteer5
Sample type RNA
 
Source name Prolonged_wakefulness_volunteer5
Organism Homo sapiens
Characteristics tissue: blood
Treatment protocol globin and DNAse treatment
Growth protocol RNA total from blood
Extracted molecule total RNA
Extraction protocol PAXgene kit
Label biotin
Label protocol IVT labeling kit
 
Hybridization protocol IVT kit (affymetrix)
Scan protocol GCOS
Description Human Genome Plus
Data processing Gene expression values were obtained using the three-step Robust Multi-array Average (RMA) pre-processing method (Irizarry et al., 2003), implemented in the Affymetrix Inc. package from R/Bioconductor. We applied the ComBat method prior to analysis for adjusting data for undesirable experimental variation (or batch effect) caused by different days in which the microarrays were processed. ComBat implements an empirical Bayes approach, that is commonly used in microarray analyses due to its ability to robustly handle high-dimensional data when sample sizes are small (Johnson et al, 2007).
 
Submission date Apr 30, 2012
Last update date May 02, 2012
Contact name Renata Pellegrino
E-mail(s) renata.pellegrino@gmail.com
Phone 2154327670
Organization name UNIFESP
Street address Rua NApoleao de Barros 925
City Sao Paulo
ZIP/Postal code 04024-002
Country Brazil
 
Platform ID GPL570
Series (1)
GSE37667 The peripheral genome-wide gene expression profiles in humans after prolonged wakefulness and sleep recovery

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1007_s_at 6.38210477
1053_at 6.789252772
117_at 9.084479444
121_at 7.002599059
1255_g_at 2.169301702
1294_at 8.089975746
1316_at 5.028128765
1320_at 2.569286832
1405_i_at 11.71868006
1431_at 2.57598467
1438_at 4.404131715
1487_at 7.462931545
1494_f_at 4.380391586
1552256_a_at 6.280617484
1552257_a_at 7.133566317
1552258_at 4.85142583
1552261_at 3.092054828
1552263_at 8.427702325
1552264_a_at 8.933802387
1552266_at 2.36949292

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM925236_Le_privado.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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