|
| Status |
Public on Apr 30, 2012 |
| Title |
PSU_ChipSeq_G1E-ER4_GATA2_(SC-9008) |
| Sample type |
SRA |
| |
|
| Source name |
G1E-ER4
|
| Organism |
Mus musculus |
| Characteristics |
lab: PSU-m
lab description: Hardison - Penn State University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: G1E-ER4
cell organism: mouse
cell description: Gata1 restored erythroid cells, differentiation induced by estradiol (E2)
cell sex: M
antibody: GATA2_(SC-9008)
antibody antibodydescription: Rabbit polyclonal antibody raised against amino acids 120-235 of GATA-2 of human origin. Antibody Target: GATA2
antibody targetdescription: The GATA family of transcription factors share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA1 is erythroid-specific and is essential for normal erythropeisis. By comparison, GATA2 is expressed at high levels in hematopoeitic progenitors, including early erythroid cells, but is strong repressed during erythroblast differentiaion.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-9008
age: E0
age description: Embryonic day 0 (stem cell)
control: Input
control description: Control signal which may be subtracted from experimental raw signal before peaks are called.
controlid: wgEncodeEM001921
replicate: 1
strain: 129
strain description: Strain 129, has widely available embryonic stem cells
|
| Biomaterial provider |
Mitch Weiss lab
|
| Treatment protocol |
diffProtD_24hr
|
| Growth protocol |
G1E_ER4_E2_Hardison_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodePsuTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodePsuTfbs
|
| |
|
| Submission date |
Apr 27, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (3) |
| GSE36029 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/PSU |
| GSE49847 |
A comparative encyclopedia of DNA elements in the mouse genome |
| GSE51338 |
Dynamic shifts in occupancy by TAL1 are guided by GATA factors and drive large-scale reprogramming of gene expression during hematopoiesis |
|
| Relations |
| SRA |
SRX145068 |
| BioSample |
SAMN00990273 |
| Named Annotation |
GSM923588_mm9_wgEncodePsuTfbsG1eer4e2Gata2sc9008ME0S129InputRepSignalRep1.bigWig |
| Named Annotation |
GSM923588_mm9_wgEncodePsuTfbsG1eer4e2Gata2sc9008ME0S129InputRepSignalRep2.bigWig |
| Named Annotation |
GSM923588_mm9_wgEncodePsuTfbsG1eer4e2Gata2sc9008ME0S129InputSig.bigWig |
