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Sample GSM922325 Query DataSets for GSM922325
Status Public on Jan 01, 2014
Title MAP2-TKO_1
Sample type RNA
Source name Cortical explant Rb-TKO cells labeled by pMAP2-Cre
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: cortical explant cell
genotype/variation: Rb-TKO
labeled by: pMAP2-Cre
Extracted molecule total RNA
Extraction protocol 10 ng of the total RNA was purified by RNeasy Micro (QIAGEN)
Label Cy3
Label protocol Total RNA was amplified following One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling), ver 6.0.
Hybridization protocol The amplified cRNA (1.5 µg) was hybridized on Agilent Whole mouse Genome Array (4 × 44K) ver 2.0 for 17 hours.
Scan protocol The Chips were scanned on a Agilent DNA Microarray Scanner using Feature Extraction software ver (Agilent).
Description Cortical explant Rb-TKO cells labeled by pMAP2-Cre
Data processing Normalized by pergentile shift (75%) and baseline to median of all samples; GeneSpring software ver 11.5.
Entities were filtered based on their signal intensity value (40-100%), and then the standard deviation (< 0.1). Filtered data linked as supplementary file on Series record.
Submission date Apr 25, 2012
Last update date Jan 01, 2014
Contact name Itsuki Ajioka
Organization name Tokyo Medical and Dental University
Department Center for Brain Integration Research
Street address 1-5-45 Yushima, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
Platform ID GPL11202
Series (1)
GSE37577 Gene expression profile of cerebral cortical cells after Rb family inactivation in progenitors and neurons

Data table header descriptions
VALUE normalized signal intensity

Data table
GE_BrightCorner -0.22430182
DarkCorner 0.14453793
A_55_P1989846 -0.5239501
A_55_P1991598 0.06908655
A_55_P2022211 -1.3608994
A_55_P1980764 -0.06143284
A_55_P1964375 0.19595194
A_51_P128876 -1.8088136
A_55_P2121042 0.06622219
A_52_P219230 0.06599474
A_51_P207591 0.071446896
A_55_P2131920 -0.5141108
A_55_P2404223 0.13213038
A_55_P2101944 -0.040310383
A_52_P358860 -0.021910429
A_51_P119031 0.044858932
A_51_P309854 -0.001526833
A_51_P343900 0.07650089
A_51_P234359 0.3722453
A_51_P487813 -0.7531085

Total number of rows: 39485

Table truncated, full table size 965 Kbytes.

Supplementary file Size Download File type/resource
GSM922325_10_mTKO1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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