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Sample GSM922324 Query DataSets for GSM922324
Status Public on Jan 01, 2014
Title MAP2-Cont_3
Sample type RNA
Source name Cortical explant control cells labeled by pMAP2-Cre
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: cortical explant cell
genotype/variation: control
labeled by: pMAP2-Cre
Extracted molecule total RNA
Extraction protocol 10 ng of the total RNA was purified by RNeasy Micro (QIAGEN)
Label Cy3
Label protocol Total RNA was amplified following One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling), ver 6.0.
Hybridization protocol The amplified cRNA (1.5 µg) was hybridized on Agilent Whole mouse Genome Array (4 × 44K) ver 2.0 for 17 hours.
Scan protocol The Chips were scanned on a Agilent DNA Microarray Scanner using Feature Extraction software ver (Agilent).
Description Cortical explant control cells labeled by pMAP2-Cre
Data processing Normalized by pergentile shift (75%) and baseline to median of all samples; GeneSpring software ver 11.5.
Entities were filtered based on their signal intensity value (40-100%), and then the standard deviation (< 0.1). Filtered data linked as supplementary file on Series record.
Submission date Apr 25, 2012
Last update date Jan 01, 2014
Contact name Itsuki Ajioka
Organization name Tokyo Medical and Dental University
Department Center for Brain Integration Research
Street address 1-5-45 Yushima, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
Platform ID GPL11202
Series (1)
GSE37577 Gene expression profile of cerebral cortical cells after Rb family inactivation in progenitors and neurons

Data table header descriptions
VALUE normalized signal intensity

Data table
GE_BrightCorner 0.15445948
DarkCorner 0.59569645
A_55_P1989846 0.0810709
A_55_P1991598 0.78049994
A_55_P2022211 0.4461317
A_55_P1980764 0.38150263
A_55_P1964375 0.22914934
A_51_P128876 0.033191204
A_55_P2121042 0.7559128
A_52_P219230 0.7500329
A_51_P207591 0.6036601
A_55_P2131920 0.16916585
A_55_P2404223 0.22874475
A_55_P2101944 0.6377177
A_52_P358860 0.3347423
A_51_P119031 -0.45942974
A_51_P309854 0.7887621
A_51_P343900 -0.076330185
A_51_P234359 0.09342766
A_51_P487813 0.56531763

Total number of rows: 39485

Table truncated, full table size 949 Kbytes.

Supplementary file Size Download File type/resource
GSM922324_09_mCont3.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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