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Sample GSM922323 Query DataSets for GSM922323
Status Public on Jan 01, 2014
Title MAP2-Cont_2
Sample type RNA
Source name Cortical explant control cells labeled by pMAP2-Cre
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: cortical explant cell
genotype/variation: control
labeled by: pMAP2-Cre
Extracted molecule total RNA
Extraction protocol 10 ng of the total RNA was purified by RNeasy Micro (QIAGEN)
Label Cy3
Label protocol Total RNA was amplified following One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling), ver 6.0.
Hybridization protocol The amplified cRNA (1.5 µg) was hybridized on Agilent Whole mouse Genome Array (4 × 44K) ver 2.0 for 17 hours.
Scan protocol The Chips were scanned on a Agilent DNA Microarray Scanner using Feature Extraction software ver (Agilent).
Description Cortical explant control cells labeled by pMAP2-Cre
Data processing Normalized by pergentile shift (75%) and baseline to median of all samples; GeneSpring software ver 11.5.
Entities were filtered based on their signal intensity value (40-100%), and then the standard deviation (< 0.1). Filtered data linked as supplementary file on Series record.
Submission date Apr 25, 2012
Last update date Jan 01, 2014
Contact name Itsuki Ajioka
Organization name Tokyo Medical and Dental University
Department Center for Brain Integration Research
Street address 1-5-45 Yushima, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
Platform ID GPL11202
Series (1)
GSE37577 Gene expression profile of cerebral cortical cells after Rb family inactivation in progenitors and neurons

Data table header descriptions
VALUE normalized signal intensity

Data table
GE_BrightCorner 0.5848336
DarkCorner 1.1735058
A_55_P1989846 0.17887115
A_55_P1991598 1.3765893
A_55_P2022211 0.23636341
A_55_P1980764 0.08131504
A_55_P1964375 -0.11474848
A_51_P128876 -0.033191204
A_55_P2121042 1.3472223
A_52_P219230 1.3392715
A_51_P207591 1.033164
A_55_P2131920 -0.20763803
A_55_P2404223 -0.08114123
A_55_P2101944 0.55148077
A_52_P358860 -0.19232488
A_51_P119031 -0.38246632
A_51_P309854 0.89705086
A_51_P343900 -0.208992
A_51_P234359 0.34254646
A_51_P487813 -0.026800632

Total number of rows: 39485

Table truncated, full table size 944 Kbytes.

Supplementary file Size Download File type/resource
GSM922323_08_mCont2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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