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Sample GSM922321 Query DataSets for GSM922321
Status Public on Jan 01, 2014
Title CAG-TKO_3
Sample type RNA
Source name Cortical explant Rb-TKO cells labeled by pCAG-Cre
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: cortical explant cell
genotype/variation: Rb-TKO
labeled by: pCAG-Cre
Extracted molecule total RNA
Extraction protocol 10 ng of the total RNA was purified by RNeasy Micro (QIAGEN)
Label Cy3
Label protocol Total RNA was amplified following One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling), ver 6.0.
Hybridization protocol The amplified cRNA (1.5 µg) was hybridized on Agilent Whole mouse Genome Array (4 × 44K) ver 2.0 for 17 hours.
Scan protocol The Chips were scanned on a Agilent DNA Microarray Scanner using Feature Extraction software ver (Agilent).
Description Cortical explant Rb-TKO cells labeled by pCAG-Cre
Data processing Normalized by pergentile shift (75%) and baseline to median of all samples; GeneSpring software ver 11.5.
Entities were filtered based on their signal intensity value (40-100%), and then the standard deviation (< 0.1). Filtered data linked as supplementary file on Series record.
Submission date Apr 25, 2012
Last update date Jan 01, 2014
Contact name Itsuki Ajioka
Organization name Tokyo Medical and Dental University
Department Center for Brain Integration Research
Street address 1-5-45 Yushima, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
Platform ID GPL11202
Series (1)
GSE37577 Gene expression profile of cerebral cortical cells after Rb family inactivation in progenitors and neurons

Data table header descriptions
VALUE normalized signal intensity

Data table
GE_BrightCorner -0.098585606
DarkCorner -0.04251671
A_55_P1989846 0.74299335
A_55_P1991598 -0.07106304
A_55_P2022211 -0.58300495
A_55_P1980764 0.06143236
A_55_P1964375 -0.70509386
A_51_P128876 -0.65669775
A_55_P2121042 -0.06622219
A_52_P219230 -0.06599474
A_51_P207591 -0.002369881
A_55_P2131920 -0.5708697
A_55_P2404223 -0.509815
A_55_P2101944 0.43509245
A_52_P358860 -0.7735779
A_51_P119031 0.070549965
A_51_P309854 0.001526833
A_51_P343900 0.11975384
A_51_P234359 0.111424446
A_51_P487813 -0.16203165

Total number of rows: 39485

Table truncated, full table size 966 Kbytes.

Supplementary file Size Download File type/resource
GSM922321_06_cTKO3.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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