|
| Status |
Public on Jan 01, 2013 |
| Title |
CT21 Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus Liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
genotype: C57BL/6
time point: CT21
|
| Treatment protocol |
Liver tissue was harvested from 4 adult male C57Bl/6 mice (Harlan-Olac, UK) once every 3 hours on the second cycle after transfer from 12L:12DR (stably entrained for 4 weeks) to DR:DR (L, light [220 µW cm-2] and DR, dim red light [< 5 µW cm-2]) and immediately frozen and then stored at -80°C prior to use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from liver samples of C57/BL6 mice over a circadian time course, 3 biological replicate samples per time point were collected and processed individually. RNA from each individual biological replicate sample was extracted using RNeasy mini kit (Qiagen Cat# 74106) and hybridized on an Affymetrix mouse Gene ST1.0 microarray.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from mouse liver cicadian time pointCT21 Replicate 2
|
| Data processing |
The data were analyzed with Genespring GX 11.
|
| |
|
| Submission date |
Apr 18, 2012 |
| Last update date |
Mar 07, 2018 |
| Contact name |
Utham Kashyap Valekunja |
| Organization name |
Smilow Center for Translational Research
|
| Department |
Department of Systems Pharmacology & Therapeutics, Perelman School of Medicine, University of Pennsylvania
|
| Street address |
10-179/180 Lab, 3400 Civic Center Boulevard, Building 421
|
| City |
Philadelphia |
| ZIP/Postal code |
PA 19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE37387 |
Transcript levels in mouse liver |
| GSE37396 |
The histone methyltransferase MLL3 regulates genome-scale circadian transcription |
|
