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Sample GSM917397 Query DataSets for GSM917397
Status Public on Jan 01, 2013
Title CT00 Replicate 3
Sample type RNA
Source name Mus musculus Liver
Organism Mus musculus
Characteristics tissue: Liver
genotype: C57BL/6
time point: CT00
Treatment protocol Liver tissue was harvested from 4 adult male C57Bl/6 mice (Harlan-Olac, UK) once every 3 hours on the second cycle after transfer from 12L:12DR (stably entrained for 4 weeks) to DR:DR (L, light [220 µW cm-2] and DR, dim red light [< 5 µW cm-2]) and immediately frozen and then stored at -80°C prior to use.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from liver samples of C57/BL6 mice over a circadian time course, 3 biological replicate samples per time point were collected and processed individually. RNA from each individual biological replicate sample was extracted using RNeasy mini kit (Qiagen Cat# 74106) and hybridized on an Affymetrix mouse Gene ST1.0 microarray.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Standard Affymetrix protocol
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from mouse liver cicadian time pointCT00 Replicate 3
Data processing The data were analyzed with Genespring GX 11.
Submission date Apr 18, 2012
Last update date Mar 07, 2018
Contact name Utham Kashyap Valekunja
Organization name University of Pennsylvania
Department Department of Systems Pharmacology & Therapeutics, Perelman School of Medicine,
Lab 10-179/180 Smilow Center for Translational Research,
Street address 3400 Civic Center Boulevard,
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platform ID GPL6246
Series (2)
GSE37387 Transcript levels in mouse liver
GSE37396 The histone methyltransferase MLL3 regulates genome-scale circadian transcription

Data table header descriptions

Data table
10344614 0.12612629
10344616 -0.003063679
10344618 0.013055801
10344620 0.030917645
10344622 -0.25012589
10344624 0.1128521
10344633 -0.057106018
10344637 0.18634415
10344653 0.017287731
10344658 -0.0297513
10344674 -0.09074688
10344679 0.030287266
10344705 0.001163006
10344707 0.16071415
10344713 -0.024116516
10344715 0.015467167
10344717 0.31217098
10344719 0.056718826
10344721 -0.022098064
10344723 0.15524435

Total number of rows: 28815

Table truncated, full table size 586 Kbytes.

Supplementary file Size Download File type/resource
GSM917397.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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