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Sample GSM917394 Query DataSets for GSM917394
Status Public on Jan 01, 2013
Title CT00 Replicate 1
Sample type RNA
 
Source name Mus musculus Liver
Organism Mus musculus
Characteristics tissue: Liver
genotype: C57BL/6
time point: CT00
Treatment protocol Liver tissue was harvested from 4 adult male C57Bl/6 mice (Harlan-Olac, UK) once every 3 hours on the second cycle after transfer from 12L:12DR (stably entrained for 4 weeks) to DR:DR (L, light [220 µW cm-2] and DR, dim red light [< 5 µW cm-2]) and immediately frozen and then stored at -80°C prior to use.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from liver samples of C57/BL6 mice over a circadian time course, 3 biological replicate samples per time point were collected and processed individually. RNA from each individual biological replicate sample was extracted using RNeasy mini kit (Qiagen Cat# 74106) and hybridized on an Affymetrix mouse Gene ST1.0 microarray.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from mouse liver cicadian time pointCT00 Replicate 1
Data processing The data were analyzed with Genespring GX 11.
 
Submission date Apr 18, 2012
Last update date Mar 07, 2018
Contact name Utham Kashyap Valekunja
Organization name Smilow Center for Translational Research
Department Department of Systems Pharmacology & Therapeutics, Perelman School of Medicine, University of Pennsylvania
Street address 10-179/180 Lab, 3400 Civic Center Boulevard, Building 421
City Philadelphia
ZIP/Postal code PA 19104
Country USA
 
Platform ID GPL6246
Series (2)
GSE37387 Transcript levels in mouse liver
GSE37396 The histone methyltransferase MLL3 regulates genome-scale circadian transcription

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10344614 0.3206935
10344616 2.60E-04
10344618 -0.007005215
10344620 7.40E-04
10344622 -0.047151566
10344624 0.00320816
10344633 -0.029580116
10344637 -0.13394356
10344653 -0.06966543
10344658 -0.16051626
10344674 -0.06301117
10344679 -0.07571125
10344705 0.15702677
10344707 -0.09621239
10344713 -0.036834717
10344715 0.015207291
10344717 -0.14613199
10344719 -0.009922981
10344721 -0.025710106
10344723 0.061700344

Total number of rows: 28815

Table truncated, full table size 588 Kbytes.




Supplementary file Size Download File type/resource
GSM917394.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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