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Sample GSM917185 Query DataSets for GSM917185
Status Public on Jul 27, 2012
Title Primary medulloblastoma sample_MB-540
Sample type RNA
 
Source name Primary medulloblastoma
Organism Homo sapiens
Characteristics age: 9.0
gender: F
histology: Classic
subgroup: Group 4
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen medulloblastoma tissue using the Trizol method (Invitrogen) as directed by the manufacturer. RNA integrity was assessed using the Agilent 2100 Bioanalyzer prior to processing for the Gene Array. Samples with RIN values ≥7 were included in the study.
Label Biotin
Label protocol As directed by Affymetrix using 400ng total RNA as starting material.
 
Hybridization protocol As directed by Affymetrix.
Scan protocol As directed by Affymetrix.
Description MB-540
Data processing Signal estimates for Gene Level Analysis were determined using Affymetrix Expression Console (1.1) and default paramters. Samples were analyzed in three separate batches according to medulloblastoma subgroup affilliation: SHH, Group 3, and Group 4.
 
Submission date Apr 18, 2012
Last update date Jul 27, 2012
Contact name Paul A. Northcott
E-mail(s) panorthcott@gmail.com
Organization name German Cancer Research Center (DKFZ)
Street address Im Neuenheimer Feld 280
City HEIDELBERG
ZIP/Postal code 69120
Country Germany
 
Platform ID GPL11532
Series (2)
GSE37382 Subgroup specific somatic copy number aberrations in the medulloblastoma genome [mRNA]
GSE37385 Subgroup specific somatic copy number aberrations in the medulloblastoma genome

Data table header descriptions
ID_REF
VALUE RMA signal intensity.

Data table
ID_REF VALUE
7896736 7.02
7896738 3.05
7896740 2.72
7896742 10.1
7896744 7.35
7896746 9.15
7896748 5.15
7896750 4.88
7896752 9.77
7896754 9.71
7896756 5.63
7896759 7.66
7896761 5.35
7896779 6.39
7896798 5.98
7896817 5.41
7896822 7.25
7896859 4.79
7896861 3.68
7896863 5.3

Total number of rows: 33297

Table truncated, full table size 420 Kbytes.




Supplementary file Size Download File type/resource
GSM917185_HTHuGene11_092911K_MT389_MB-540.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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