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Sample GSM916822 Query DataSets for GSM916822
Status Public on Oct 10, 2013
Title Wild type mRNA 3
Sample type RNA
Source name lymphoma biopsies
Organism Homo sapiens
Characteristics genotype: Wild type
tissue: lymphoma biopsy
Growth protocol Patient Samples
Extracted molecule total RNA
Extraction protocol RNA was extracted from fresh frozen lymphoma biopsies using RNeasy mini kit (Qiagen Valenica, CA)
Label biotin
Label protocol Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer's instructions
Hybridization protocol Following fragmentation, 15 µg of cRNA were hybridized for 16hr on Affymetrix HuGene-1_0-st [transcript (gene version)] as recommended by manufacturer
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from TET2 Wild type patient sample
Data processing The data was analyzed with Gene Spring GX 12
Submission date Apr 17, 2012
Last update date Oct 10, 2013
Contact name Vasu Punj
Organization name USC
Street address Biggy Street
City Los Angeles
State/province CA
ZIP/Postal code 90033
Country USA
Platform ID GPL6244
Series (2)
GSE37363 TET2 loss-of-function mutations associate with a DNA hypermethylation signature in diffuse large B-cell lymphoma (mRNA)
GSE37365 TET2 loss-of-function mutations associate with a DNA hypermethylation signature in diffuse large B-cell lymphoma

Data table header descriptions
VALUE PLIER 16 normalized

Data table
7896736 22.591028
7896738 25.88087
7896740 23.943213
7896742 113.77233
7896744 26.340733
7896746 203.0811
7896748 230.13193
7896750 55.067966
7896752 471.77887
7896754 108.60651
7896756 54.386444
7896759 48.867558
7896761 62.752186
7896779 97.5474
7896798 48.037273
7896817 276.2449
7896822 64.5726
7896859 66.84678
7896861 19.453037
7896863 81.09906

Total number of rows: 28869

Table truncated, full table size 497 Kbytes.

Supplementary file Size Download File type/resource
GSM916822_G28-HuGene-1_0-st.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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