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Sample GSM915162 Query DataSets for GSM915162
Status Public on Apr 13, 2012
Title Caltech_ChipSeq_C2C12_USF-1_Control_50bp
Sample type SRA
 
Source name C2C12
Organism Mus musculus
Characteristics lab: Caltech-m
lab description: Wold - Califonia Institute of Technology
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: C2C12
cell organism: mouse
cell description: Myoblast cell line derived from thigh muscle of C3H mice after crush injury
cell sex: F
antibody: USF-1
antibody description: Rabbit polyclonal, USF-1 (C-20). Antibody Target: USF1
antibody targetdescription: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-229
control: Control_50bp
control description: This data represents a control being compared to experiments using read length of 50 bp
treatment: None
treatment description: No special treatment or protocol applies
age: immortalized
age description: Immortal cells
control: Control_50bp
control description: This data represents a control being compared to experiments using read length of 50 bp
controlid: 11612
labexpid: 11923
labversion: Myoblast
replicate: 1
strain: C3H
strain description: Strain C3H, Very related to C57BL/6
Biomaterial provider Barbara Wold lab
Treatment protocol None
Growth protocol C2C12_Wold_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeCaltechTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeCaltechTfbs
 
Submission date Apr 13, 2012
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9250
Series (3)
GSE36024 Transcription Factor Binding Sites by ChIP-seq from ENCODE/Caltech
GSE49847 A comparative encyclopedia of DNA elements in the mouse genome
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX142513
BioSample SAMN00857804
Named Annotation GSM915162_mm9_wgEncodeCaltechTfbsC2c12Usf1FCntrl50bPcr1xSigRep1.bigWig

Supplementary file Size Download File type/resource
GSM915162_mm9_wgEncodeCaltechTfbsC2c12Usf1FCntrl50bPcr1xPkRep1.narrowPeak.gz 139.9 Kb (ftp)(http) NARROWPEAK
GSM915162_mm9_wgEncodeCaltechTfbsC2c12Usf1FCntrl50bPcr1xSigRep1.bigWig 112.1 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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