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Sample GSM915162 Query DataSets for GSM915162
Status Public on Apr 13, 2012
Title Caltech_ChipSeq_C2C12_USF-1_Control_50bp
Sample type SRA
Source name C2C12
Organism Mus musculus
Characteristics lab: Caltech-m
lab description: Wold - Califonia Institute of Technology
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: C2C12
cell organism: mouse
cell description: Myoblast cell line derived from thigh muscle of C3H mice after crush injury
cell sex: F
antibody: USF-1
antibody description: Rabbit polyclonal, USF-1 (C-20). Antibody Target: USF1
antibody targetdescription: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-229
control: Control_50bp
control description: This data represents a control being compared to experiments using read length of 50 bp
treatment: None
treatment description: No special treatment or protocol applies
age: immortalized
age description: Immortal cells
control: Control_50bp
control description: This data represents a control being compared to experiments using read length of 50 bp
controlid: 11612
labexpid: 11923
labversion: Myoblast
replicate: 1
strain: C3H
strain description: Strain C3H, Very related to C57BL/6
Biomaterial provider Barbara Wold lab
Treatment protocol None
Growth protocol C2C12_Wold_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
Data processing
Submission date Apr 13, 2012
Last update date May 15, 2019
Contact name ENCODE DCC
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platform ID GPL9250
Series (3)
GSE36024 Transcription Factor Binding Sites by ChIP-seq from ENCODE/Caltech
GSE49847 A comparative encyclopedia of DNA elements in the mouse genome
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
SRA SRX142513
BioSample SAMN00857804
Named Annotation GSM915162_mm9_wgEncodeCaltechTfbsC2c12Usf1FCntrl50bPcr1xSigRep1.bigWig

Supplementary file Size Download File type/resource
GSM915162_mm9_wgEncodeCaltechTfbsC2c12Usf1FCntrl50bPcr1xPkRep1.narrowPeak.gz 139.9 Kb (ftp)(http) NARROWPEAK
GSM915162_mm9_wgEncodeCaltechTfbsC2c12Usf1FCntrl50bPcr1xSigRep1.bigWig 112.1 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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