|
Status |
Public on Apr 13, 2012 |
Title |
Caltech_ChipSeq_C2C12_USF-1_Control_50bp |
Sample type |
SRA |
|
|
Source name |
C2C12
|
Organism |
Mus musculus |
Characteristics |
lab: Caltech-m lab description: Wold - Califonia Institute of Technology datatype: ChipSeq datatype description: Chromatin IP Sequencing cell: C2C12 cell organism: mouse cell description: Myoblast cell line derived from thigh muscle of C3H mice after crush injury cell sex: F antibody: USF-1 antibody description: Rabbit polyclonal, USF-1 (C-20). Antibody Target: USF1 antibody targetdescription: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types. antibody vendorname: Santa Cruz Biotech antibody vendorid: sc-229 control: Control_50bp control description: This data represents a control being compared to experiments using read length of 50 bp treatment: None treatment description: No special treatment or protocol applies age: immortalized age description: Immortal cells control: Control_50bp control description: This data represents a control being compared to experiments using read length of 50 bp controlid: 11612 labexpid: 11923 labversion: Myoblast replicate: 1 strain: C3H strain description: Strain C3H, Very related to C57BL/6
|
Biomaterial provider |
Barbara Wold lab
|
Treatment protocol |
None
|
Growth protocol |
C2C12_Wold_protocol.pdf
|
Extracted molecule |
genomic DNA |
Extraction protocol |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeCaltechTfbs
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer II |
|
|
Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeCaltechTfbs
|
|
|
Submission date |
Apr 13, 2012 |
Last update date |
May 15, 2019 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL9250 |
Series (3) |
GSE36024 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/Caltech |
GSE49847 |
A comparative encyclopedia of DNA elements in the mouse genome |
GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
Relations |
SRA |
SRX142513 |
BioSample |
SAMN00857804 |
Named Annotation |
GSM915162_mm9_wgEncodeCaltechTfbsC2c12Usf1FCntrl50bPcr1xSigRep1.bigWig |