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Sample GSM912909 Query DataSets for GSM912909
Status Public on Apr 11, 2012
Title Stanford_ChipSeq_CH12_CTCF_(SC-15914)_IgG-rab
Sample type SRA
Source name CH12
Organism Mus musculus
Characteristics lab: Stanford-m
lab description: Snyder - Stanford University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: CH12
cell organism: mouse
cell description: B-cell lymphoma (GM12878 analog)
cell sex: F
antibody: CTCF_(SC-15914)
antibody antibodydescription: Goat polyclonal, epitope mapping near the C-terminus of CTCF of human origin. Antibody Target: CTCF
antibody targetdescription: Transcriptional regulator with 11 highly conserved zinc finger domains. Depending on context, can bind a histone acetyltransferase (HAT)-containing complex and function as transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as transcriptional repressor. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation. Can dimerize, mediating long-range chromatin looping. When bound to chromatin, provides an anchor point for nucleosomes positioning. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-15914
treatment: None
treatment description: No special treatment or protocol applies
control: IgG-rab
control description: Input signal from Normal Rabbit IgG ChIP-seq.
age: immortalized
age description: Immortal cells
control: IgG-rab
control description: Input signal from Normal Rabbit IgG ChIP-seq.
controlid: CH12/None/Input/IgG-rab
replicate: 1
strain: B10.H-2aH-4bp/Wts
strain description: Derived by inbreeding from selected F2 progeny of B10.A X B10.129
Biomaterial provider Weissman lab
Treatment protocol None
Extracted molecule genomic DNA
Extraction protocol
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
Data processing
Submission date Apr 11, 2012
Last update date May 15, 2019
Contact name ENCODE DCC
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platform ID GPL9250
Series (3)
GSE36030 Transcription Factor Binding Sites by ChIP-seq from ENCODE/Stanford/Yale
GSE49847 A comparative encyclopedia of DNA elements in the mouse genome
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
SRA SRX140372
BioSample SAMN00854832
Named Annotation GSM912909_mm9_wgEncodeSydhTfbsCh12CtcfbIggrabSig.bigWig

Supplementary file Size Download File type/resource
GSM912909_mm9_wgEncodeSydhTfbsCh12CtcfbIggrabPk.narrowPeak.gz 1.5 Mb (ftp)(http) NARROWPEAK
GSM912909_mm9_wgEncodeSydhTfbsCh12CtcfbIggrabSig.bigWig 289.6 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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