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Sample GSM911078 Query DataSets for GSM911078
Status Public on Nov 12, 2012
Title Identification of miR-200a target genes in renal cell carcinoma, caki-1
Sample type RNA
 
Channel 1
Source name control miRNA transfected cell line (A498)
Organism Homo sapiens
Characteristics cell line: caki-1
cell type: renal cell carcinoma
transfected mirna or sirna: control
Treatment protocol microRNA transfection using Lipofectamine RNiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or DMEM.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol following the manufacturer's instructions.
Label Cy3
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
Channel 2
Source name miR-200a transfected cell line (caki-1)
Organism Homo sapiens
Characteristics cell line: A498
cell type: renal cell carcinoma
transfected mirna or sirna: miR-200a
Treatment protocol microRNA transfection using Lipofectamine RNiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or DMEM.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol following the manufacturer's instructions.
Label Cy5
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
 
Hybridization protocol Agilent two-color gene expression hyb/wash protocol
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 9.5.3
Description Differentially expressed genes by miR-200a transfection.
Data processing LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
 
Submission date Apr 09, 2012
Last update date Nov 12, 2012
Contact name Naohiko Seki
E-mail(s) naoseki@faculty.chiba-u.jp
Phone 81-43-226-2971
Fax 81-43-227-3442
Organization name Chiba University
Department School of Medicine
Lab Functional Genomics
Street address 1-8-1 Inohana,Chuo-ku
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL13607
Series (1)
GSE37119 Identification of target genes of cancer-related microRNAs in human cancer

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 -2.071364862e-002
2 0.000000000e+000
3 0.000000000e+000
4 -5.102613049e-002
5 6.267360564e-002
6 1.246720816e-001
7 6.436904260e-003
8 3.496041327e-002
9 0.000000000e+000
10 5.346028939e-001
11 0.000000000e+000
12 -2.272314483e-002
13 -2.617932820e-004
14 6.363049260e-002
15 -1.861846519e-002
16 0.000000000e+000
17 -1.854142146e-002
18 0.000000000e+000
19 0.000000000e+000
20 3.650050367e-002

Total number of rows: 62976

Table truncated, full table size 1428 Kbytes.




Supplementary file Size Download File type/resource
GSM911078_A1-caki1-200a_caki1-C-4.txt.gz 21.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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