|
| Status |
Public on Jan 28, 2013 |
| Title |
myoblast_LMNA_wt |
| Sample type |
SRA |
| |
|
| Source name |
myoblast
|
| Organism |
Mus musculus |
| Characteristics |
sample type: myoblast_LMNA_wt
tissue: myoblast
strain: C57Bl/6
genotype/variation: wt
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated by the TRIZOL method and purified with RNeasy kit. NUGEN ovation RNA-Seq (samples 1-6) and NUGEN complete RNA-Seq protocol (samples 7-12)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
demultiplexing and barcode clipping
quality filtering
mapping with tophat1.2.0, USCS known genes, mm9
cuffdiff0.0.5
Genome_build: mm9
Supplementary_files_format_and_content: fpkm_values.xls;normalized expression values used for assessment of differential gene expression, only one fpkm value is calculated per sample type
|
| |
|
| Submission date |
Apr 06, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Stefan Krebs |
| E-mail(s) |
krebs-st@lmb.uni-muenchen.de
|
| Phone |
0049-89-2180 76715
|
| Organization name |
Ludwig-Maximilian University
|
| Department |
Gene Center
|
| Lab |
Lafuga Genomics
|
| Street address |
Feodor-Lynen-Str. 25
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11002 |
| Series (1) |
| GSE37076 |
Lamin and Lamin B receptor knockout |
|
| Relations |
| SRA |
SRX136610 |
| BioSample |
SAMN00849848 |
