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Sample GSM896031 Query DataSets for GSM896031
Status Public on Dec 01, 2012
Title LERKO biological rep 2
Sample type RNA
Source name Liver selective ERalpha knockout mouse
Organism Mus musculus
Characteristics Sex: female
genotype: Liver selective ERalpha knockout mouse
tissue: liver
genetic background: C57BL/6J
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from frozen mouse tissues using the Trizol reagent (Invitrogen) as per manufacturer’s instructions. The isolated RNA was subsequently purified with the RNeasy Plus Mini Kits (Qiagen),
Label biotin
Label protocol Fragmented single-stranded sense DNA was end labeled with biotin according to the Affymetrx Gene chip WT Terminal Labeling and hybridization user manual
Hybridization protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was hybridized in the Affymetrix Gene Titan Instrument standard procedure
Scan protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was scanned in Affymetrix Gene Titan Instrument standard procedure
Data processing The data were analyzed with oilgo package (version 1.16.0) in Bioconductor. Transcripts level were summaried for core probesets using RMA.
Submission date Mar 14, 2012
Last update date Dec 01, 2012
Contact name Marko Matic
Organization name Karolinska Institutet
Street address Halsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platform ID GPL11533
Series (1)
GSE36514 Estrogen signaling and the Metabolic Syndrome: Targeting the hepatic ERalpha action

Data table header descriptions
VALUE log2 RMA signal

Data table
10338001 11.31568569
10338002 3.923409994
10338003 9.558705896
10338004 8.90519365
10338005 1.834709499
10338006 2.033627746
10338007 2.260066548
10338008 2.505307661
10338009 5.95628951
10338010 1.862969254
10338011 3.395458666
10338012 1.934130297
10338013 1.677237025
10338014 1.73194862
10338015 1.666673323
10338016 4.818438909
10338017 12.7636836
10338018 4.233246579
10338019 3.064830962
10338020 5.740337715

Total number of rows: 35556

Table truncated, full table size 725 Kbytes.

Supplementary file Size Download File type/resource
GSM896031_MM11_C07.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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