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Sample GSM896030 Query DataSets for GSM896030
Status Public on Dec 01, 2012
Title LERKO biological rep 1
Sample type RNA
Source name Liver selective ERalpha knockout mouse
Organism Mus musculus
Characteristics Sex: female
genotype: Liver selective ERalpha knockout mouse
tissue: liver
genetic background: C57BL/6J
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from frozen mouse tissues using the Trizol reagent (Invitrogen) as per manufacturer’s instructions. The isolated RNA was subsequently purified with the RNeasy Plus Mini Kits (Qiagen),
Label biotin
Label protocol Fragmented single-stranded sense DNA was end labeled with biotin according to the Affymetrx Gene chip WT Terminal Labeling and hybridization user manual
Hybridization protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was hybridized in the Affymetrix Gene Titan Instrument standard procedure
Scan protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was scanned in Affymetrix Gene Titan Instrument standard procedure
Data processing The data were analyzed with oilgo package (version 1.16.0) in Bioconductor. Transcripts level were summaried for core probesets using RMA.
Submission date Mar 14, 2012
Last update date Dec 01, 2012
Contact name Marko Matic
Organization name Karolinska Institutet
Street address Halsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platform ID GPL11533
Series (1)
GSE36514 Estrogen signaling and the Metabolic Syndrome: Targeting the hepatic ERalpha action

Data table header descriptions
VALUE log2 RMA signal

Data table
10338001 11.17979317
10338002 4.040904228
10338003 9.454557253
10338004 8.664609312
10338005 1.834814728
10338006 2.075828207
10338007 2.350586838
10338008 2.613503103
10338009 6.032800864
10338010 1.838250606
10338011 3.553922941
10338012 1.945753898
10338013 1.670387342
10338014 1.750502282
10338015 1.641228296
10338016 4.883706699
10338017 12.52798285
10338018 4.480963466
10338019 3.213561012
10338020 5.746004039

Total number of rows: 35556

Table truncated, full table size 725 Kbytes.

Supplementary file Size Download File type/resource
GSM896030_MM10_B07.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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