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Sample GSM896028 Query DataSets for GSM896028
Status Public on Dec 01, 2012
Title Control biological rep 2
Sample type RNA
 
Source name Control mouse
Organism Mus musculus
Characteristics Sex: female
genotype: Control mouse
tissue: liver
genetic background: C57BL/6J
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from frozen mouse tissues using the Trizol reagent (Invitrogen) as per manufacturer’s instructions. The isolated RNA was subsequently purified with the RNeasy Plus Mini Kits (Qiagen),
Label biotin
Label protocol Fragmented single-stranded sense DNA was end labeled with biotin according to the Affymetrx Gene chip WT Terminal Labeling and hybridization user manual
 
Hybridization protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was hybridized in the Affymetrix Gene Titan Instrument standard procedure
Scan protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was scanned in Affymetrix Gene Titan Instrument standard procedure
Data processing The data were analyzed with oilgo package (version 1.16.0) in Bioconductor. Transcripts level were summaried for core probesets using RMA.
 
Submission date Mar 14, 2012
Last update date Dec 01, 2012
Contact name Marko Matic
Organization name Karolinska Institutet
Street address Halsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
 
Platform ID GPL11533
Series (1)
GSE36514 Estrogen signaling and the Metabolic Syndrome: Targeting the hepatic ERalpha action

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
10338001 11.17424108
10338002 3.905804331
10338003 9.403829835
10338004 8.759670348
10338005 1.875970254
10338006 2.056857352
10338007 2.336320905
10338008 2.569113293
10338009 5.796712822
10338010 1.885921396
10338011 3.447425803
10338012 1.967103393
10338013 1.657753057
10338014 1.764643844
10338015 1.682424708
10338016 4.674027419
10338017 12.49494355
10338018 4.198703273
10338019 3.153589566
10338020 5.569299867

Total number of rows: 35556

Table truncated, full table size 725 Kbytes.




Supplementary file Size Download File type/resource
GSM896028_MM8_H05.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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