|
Status |
Public on Feb 24, 2013 |
Title |
PT12 tamoxifen treated, replicate 1 |
Sample type |
RNA |
|
|
Source name |
Tumor
|
Organism |
Homo sapiens |
Characteristics |
xenograft: PT12 treatment: tamoxifen treated
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 3.5 ug of cDNA were hybridized for 20 hr at 48C on HuGene 1.1ST Array strip. GeneStrips were washed and stained in the Affymetrix Gene Atlas fluidics station.
|
Scan protocol |
GeneStrips were scanned using the Gene Atlas imaging station.
|
Description |
Human breast cancer was xenografted in NOD/SCID mice
|
Data processing |
The data were analyzed with Partek using RMA as normalization method.
|
|
|
Submission date |
Feb 24, 2012 |
Last update date |
Feb 24, 2013 |
Contact name |
peter kabos |
E-mail(s) |
peter.kabos@ucdenver.edu
|
Organization name |
University of Colorado Denver
|
Department |
Medicine
|
Street address |
12801 E. 17th Avenue
|
City |
Aurora |
ZIP/Postal code |
80045 |
Country |
USA |
|
|
Platform ID |
GPL11532 |
Series (1) |
GSE36075 |
Estrogen receptor dynamics in diverse patient-derived luminal breast cancer xenografts |
|