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Sample GSM880768 Query DataSets for GSM880768
Status Public on Feb 24, 2013
Title PT12 estrogen withdrawal, replicate 1
Sample type RNA
 
Source name Tumor
Organism Homo sapiens
Characteristics xenograft: PT12
treatment: estrogen withdrawal
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 3.5 ug of cDNA were hybridized for 20 hr at 48C on HuGene 1.1ST Array strip. GeneStrips were washed and stained in the Affymetrix Gene Atlas fluidics station.
Scan protocol GeneStrips were scanned using the Gene Atlas imaging station.
Description Human breast cancer was xenografted in NOD/SCID mice
Data processing The data were analyzed with Partek using RMA as normalization method.
 
Submission date Feb 24, 2012
Last update date Feb 24, 2013
Contact name peter kabos
E-mail(s) peter.kabos@ucdenver.edu
Organization name University of Colorado Denver
Department Medicine
Street address 12801 E. 17th Avenue
City Aurora
ZIP/Postal code 80045
Country USA
 
Platform ID GPL11532
Series (1)
GSE36075 Estrogen receptor dynamics in diverse patient-derived luminal breast cancer xenografts

Data table header descriptions
ID_REF
VALUE RMA values

Data table
ID_REF VALUE
7892501 2.61546
7892502 3.19628
7892503 2.30736
7892504 7.16342
7892505 2.27833
7892506 3.12371
7892507 3.77365
7892508 3.22159
7892509 9.46737
7892510 3.66294
7892511 3.22124
7892512 7.02209
7892513 2.68062
7892514 10.9796
7892515 8.05571
7892516 2.60734
7892517 3.90632
7892518 2.21582
7892519 5.47344
7892520 9.25102

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM880768_115-22_LT.ga.cel.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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