|Public on Feb 24, 2013
|PE4 estrogen treated, replicate 3
treatment: estrogen treated
|Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|Following fragmentation, 3.5 ug of cDNA were hybridized for 20 hr at 48C on HuGene 1.1ST Array strip. GeneStrips were washed and stained in the Affymetrix Gene Atlas fluidics station.
|GeneStrips were scanned using the Gene Atlas imaging station.
|Human breast cancer was xenografted in NOD/SCID mice
|The data were analyzed with Partek using RMA as normalization method.
|Feb 24, 2012
|Last update date
|Feb 24, 2013
|University of Colorado Denver
|12801 E. 17th Avenue
|Estrogen receptor dynamics in diverse patient-derived luminal breast cancer xenografts