|
Status |
Public on Feb 22, 2012 |
Title |
maternal whole blood replicate 2 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
methylated CpG-island recovery assay (MIRA) enriched, maternal whole blood, 2 of 4 mother-baby pairs
|
Organism |
Homo sapiens |
Characteristics |
sample id: NMB6 tissue: maternal whole blood gender: Female sample type: methylation-enriched DNA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated from fresh frozen tissues using standard phenol/chloroform method.
|
Label |
cy5
|
Label protocol |
The products of whole genome amplification from the total input DNA without MBD enrichment and from methylation-enriched DNA were purified by using DNA purification kit, respectively. The purified DNA fragments were then labeled with cy3-dCTP or cy5-dCTP with Klenow enzyme (Takara) according to the instruction of the manufacturer.
|
|
|
Channel 2 |
Source name |
input, maternal whole blood, 2 of 4 mother-baby pairs
|
Organism |
Homo sapiens |
Characteristics |
sample id: NMB6 tissue: maternal whole blood gender: Female sample type: total input DNA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated from fresh frozen tissues using standard phenol/chloroform method.
|
Label |
cy3
|
Label protocol |
The products of whole genome amplification from the total input DNA without MBD enrichment and from methylation-enriched DNA were purified by using DNA purification kit, respectively. The purified DNA fragments were then labeled with cy3-dCTP or cy5-dCTP with Klenow enzyme (Takara) according to the instruction of the manufacturer.
|
|
|
|
Hybridization protocol |
According to the instructions of Agilent Oligo aCGH Hybridization Kit
|
Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
Description |
Sample name: NMB6-blo_cy5/cy3 Biological replicate 2 of 4 maternal whole blood US10283824_252984210018_S01_CGH_107_Sep09_2_2.txt
|
Data processing |
Agilent GeneSpring GX 11.5 was used for background subtraction and LOWESS normalization.Following global mean normalization, probes with an intensity <400 were discarded for further analysis.
|
|
|
Submission date |
Feb 22, 2012 |
Last update date |
Feb 22, 2012 |
Contact name |
wang liang |
E-mail(s) |
wangliangkexue@163.com
|
Organization name |
BioChainBJ
|
Street address |
7A North Yongchang Road BDA
|
City |
Beijing |
ZIP/Postal code |
100176 |
Country |
China |
|
|
Platform ID |
GPL14835 |
Series (1) |
GSE35997 |
DNA hypermethylation in placental tissues: placental tissues vs. maternal peripheral bloods |
|