Data table header descriptions 
ID_REF 

Ratio 
the ratio of the median intensities of each feature for each wavelength, with the median background subtracted. 
F635 Median 
median intensity in foreground (Cy5 channel) 
F635 Mean 
mean intensity in foreground (Cy5 channel) 
F635 SD 
the standard deviation of the feature pixel intensity at wavelength #1 (635 nm) 
B635 Median 
the median feature background intensity at wavelength #1 (635 nm) 
B635 Mean 
the mean feature background intensity at wavelength #1 (635 nm) 
B635 SD 
the standard deviation of the feature background intensity at wavelength #1 (635 nm) 
% > B635+1SD 
the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm). 
% > B635+2SD 
the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm). 
F635 % Sat. 
the percentage of feature pixels at wavelength #1 that are saturated 
F532 Median 
median feature pixel intensity at wavelength #2 (532 nm) 
F532 Mean 
mean feature pixel intensity at wavelength #2 (532 nm) 
F532 SD 
the standard deviation of the feature intensity at wavelength #2 (532 nm) 
B532 Median 
the median feature background intensity at wavelength #2 (532 nm) 
B532 Mean 
mean feature pixel intensity at wavelength #2 (532 nm). 
B532 SD 
the standard deviation of the feature intensity at wavelength #2 (532 nm). 
% > B532+1SD 
the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm). 
% > B532+2SD 
the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm). 
F532 % Sat. 
the percentage of feature pixels at wavelength #2 that are saturated. 
Ratio of Means 
the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted. 
Median of Ratios 
the median of pixelbypixel ratios of pixel intensities, with the median background subtracted. 
Mean of Ratios 
the geometric mean of the pixelbypixel ratios of pixel intensities, with the median background subtracted. 
Ratios SD 
the geometric standard deviation of the pixel intensity ratios. 
Rgn Ratio 
the regression ratio of every pixel in a 2featurediameter circle around the center of the feature. 
Rgn R? 
the coefficient of determination for the current regression value. 
F Pixels 
the total number of feature pixels. 
B Pixels 
the total number of background pixels. 
Sum of Medians 
the sum of the median intensities for each wavelength, with the median background subtracted. 
Sum of Means 
the sum of the arithmetic mean intensities for each wavelength, with the median background subtracted. 
VALUE 
same as UNF_VALUE but with flagged values removed 
F635 Median  B635 
the median feature pixel intensity at wavelength #1 with the median background subtracted. 
F532 Median  B532 
the median feature pixel intensity at wavelength #2 with the median background subtracted. 
F635 Mean  B635 
the mean feature pixel intensity at wavelength #1 with the median background subtracted. 
F532 Mean  B532 
the mean feature pixel intensity at wavelength #2 with the median background subtracted. 
Flags 
the type of flag associated with a feature. 
UNF_VALUE 
log (base 2) transform of the ratio of the medians. 