|
Status |
Public on Jan 16, 2025 |
Title |
TR-Sc-2 |
Sample type |
SRA |
|
|
Source name |
ADSCs
|
Organism |
Mus musculus |
Characteristics |
cell type: ADSCs genotype: WT treatment: Scrambled batch: batch 2
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol (Takara). 800 ug of total RNA was used for the construction of sequencing libraries. The extracted mRNA is enriched using mRNA Capture Beads. After purification with beads, the mRNA is fragmented using high temperatures. The fragmented mRNA is then used as a template to synthesize the first strand of cDNA in a reverse transcription enzyme mixture system. While synthesizing the second strand of cDNA, end repair and A-tailing are completed. Next, adapters are ligated, and Hieff NGSĀ® DNA Selection Beads are used for purification to select target fragments.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Library name: Sample 2 TR-Sc-2 in FPKM_shifit1ADSC.txt
|
Data processing |
Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork (parameters - mismatch cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8) Assembly: NCBI_GCF_000001635.27_GRCm39 Supplementary files format and content: tab-delimited text file includes TPM for each Sample
|
|
|
Submission date |
Jan 08, 2025 |
Last update date |
Jan 16, 2025 |
Contact name |
Lei Hou |
E-mail(s) |
dr_houlei@shsmu.edu.cn
|
Phone |
+8618117283090
|
Organization name |
Songjiang Hospital and Songjiang Research Institute, School of Medicine, Shanghai Jiao Tong University
|
Department |
Department of Cardiology
|
Street address |
746 Zhongshan Middle Road
|
City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
201699 |
Country |
China |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE286132 |
IFIT1 Knockdown Mitigates Translational Suppression, Enhancing Myogenic Differentiation and Revitalizing Mesenchymal Stem Cell Therapy for Myocardial Infarction [II] |
|
Relations |
BioSample |
SAMN46167104 |
SRA |
SRX27291919 |