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Status |
Public on Feb 08, 2012 |
Title |
in vitro spleen cells 72hr IM(-)_rep2 |
Sample type |
RNA |
|
|
Source name |
spleen,72hr,IM(-), replicate 2
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: female age: 8 weeks tissue: splenocytes agent: control
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Treatment protocol |
The mouse splenocytes were cultured for 72 h with or without 10 µM Imatinib (IM) .
|
Extracted molecule |
total RNA |
Extraction protocol |
QIAGEN RNeasy kit
|
Label |
Cy3
|
Label protocol |
After fragmentation, 1650 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wash buffer 2 (Agilent)
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|
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Hybridization protocol |
1650.0 ng at 65 degree_C during 17 hours
|
Scan protocol |
Arrays were scanned using Agilent G2565AA Microarray Scanner System.
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Description |
Gene expression in spleen cells cultured without 10 µM Imatinib in conditioning medium containing IL-4 and LPS for 72 h US14702391_252665512076_S01_GE1-v5_91_0806_1_3.txt
|
Data processing |
Data processed with FeatureExrtraction v9.1. For normalization of raw microarray data, 75 percentile shift normalization was applied with GeneSpring GX v11.2.
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|
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Submission date |
Feb 06, 2012 |
Last update date |
Feb 08, 2012 |
Contact name |
toyotaka kawamata |
E-mail(s) |
toyotaka@ims.u-tokyo.ac.jp
|
Organization name |
The Institute of Medical Science,The University of Tokyo
|
Department |
Hematology-Oncology/Molecular therapy
|
Street address |
4-6-1,Shirokanedai,Minato-ku
|
City |
Tokyo |
ZIP/Postal code |
108-8639 |
Country |
Japan |
|
|
Platform ID |
GPL11202 |
Series (1) |
GSE35559 |
Gene expression profile induced by Imatinib |
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