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Sample GSM870530 Query DataSets for GSM870530
Status Public on Feb 08, 2012
Title in vitro spleen cells 72hr IM(-)_rep2
Sample type RNA
Source name spleen,72hr,IM(-), replicate 2
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 8 weeks
tissue: splenocytes
agent: control
Treatment protocol The mouse splenocytes were cultured for 72 h with or without 10 µM Imatinib (IM) .
Extracted molecule total RNA
Extraction protocol QIAGEN RNeasy kit
Label Cy3
Label protocol After fragmentation, 1650 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wash buffer 2 (Agilent)
Hybridization protocol 1650.0 ng at 65 degree_C during 17 hours
Scan protocol Arrays were scanned using Agilent G2565AA Microarray Scanner System.
Description Gene expression in spleen cells cultured without 10 µM Imatinib in conditioning medium containing IL-4 and LPS for 72 h
Data processing Data processed with FeatureExrtraction v9.1. For normalization of raw microarray data, 75 percentile shift normalization was applied with GeneSpring GX v11.2.
Submission date Feb 06, 2012
Last update date Feb 08, 2012
Contact name toyotaka kawamata
Organization name The Institute of Medical Science,The University of Tokyo
Department Hematology-Oncology/Molecular therapy
Street address 4-6-1,Shirokanedai,Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
Platform ID GPL11202
Series (1)
GSE35559 Gene expression profile induced by Imatinib

Data table header descriptions
VALUE Log2 normalized signal intensity

Data table
GE_BrightCorner 4.182602
DarkCorner -4.2854757
A_55_P1989846 -0.9619303
A_55_P1991598 -8.278155
A_55_P2022211 -3.957809
A_55_P1980764 -4.442972
A_55_P1964375 1.779541
A_51_P128876 -0.40209103
A_55_P2121042 -7.0745587
A_52_P219230 -8.063308
A_51_P207591 -5.6071525
A_55_P2131920 -8.259697
A_55_P2404223 -1.8586607
A_55_P2101944 3.9943743
A_52_P358860 0.2679186
A_51_P119031 -1.0226889
A_51_P309854 -8.332809
A_51_P343900 3.8007717
A_51_P234359 -3.5055323
A_51_P487813 1.3727236

Total number of rows: 39485

Table truncated, full table size 922 Kbytes.

Supplementary file Size Download File type/resource
GSM870530.txt.gz 7.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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