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Sample GSM870528 Query DataSets for GSM870528
Status Public on Feb 08, 2012
Title in vitro spleen cells 72hr IM(+)_rep2
Sample type RNA
Source name spleen,72hr,IM(+), replicate 2
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 8 weeks
tissue: splenocytes
agent: Imatinib (IM)
Treatment protocol The mouse splenocytes were cultured for 72 h with or without 10 µM Imatinib (IM) .
Extracted molecule total RNA
Extraction protocol QIAGEN RNeasy kit
Label Cy3
Label protocol After fragmentation, 1650 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wash buffer 2 (Agilent)
Hybridization protocol 1650.0 ng at 65 degree_C during 17 hours
Scan protocol Arrays were scanned using Agilent G2565AA Microarray Scanner System.
Description Gene expression in spleen cells cultured with 10 µM Imatinib in conditioning medium containing IL-4 and LPS for 72 h
Data processing Data processed with FeatureExrtraction v9.1. For normalization of raw microarray data, 75 percentile shift normalization was applied with GeneSpring GX v11.2.
Submission date Feb 06, 2012
Last update date Feb 08, 2012
Contact name toyotaka kawamata
Organization name The Institute of Medical Science,The University of Tokyo
Department Hematology-Oncology/Molecular therapy
Street address 4-6-1,Shirokanedai,Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
Platform ID GPL11202
Series (1)
GSE35559 Gene expression profile induced by Imatinib

Data table header descriptions
VALUE Log2 normalized signal intensity

Data table
GE_BrightCorner 4.0324335
DarkCorner -5.0610013
A_55_P1989846 -0.45488358
A_55_P1991598 -8.031884
A_55_P2022211 -1.4789634
A_55_P1980764 -4.0659227
A_55_P1964375 1.5590754
A_51_P128876 -2.0624895
A_55_P2121042 -7.867882
A_52_P219230 -8.513061
A_51_P207591 -5.3558216
A_55_P2131920 -8.50934
A_55_P2404223 -1.6964788
A_55_P2101944 5.3593817
A_52_P358860 0.030500412
A_51_P119031 -1.1662502
A_51_P309854 -8.605376
A_51_P343900 3.8267345
A_51_P234359 -3.8180609
A_51_P487813 1.557765

Total number of rows: 39485

Table truncated, full table size 923 Kbytes.

Supplementary file Size Download File type/resource
GSM870528.txt.gz 7.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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