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Sample GSM870527 Query DataSets for GSM870527
Status Public on Feb 08, 2012
Title in vitro spleen cells 72hr IM(+)_rep1
Sample type RNA
Source name spleen,72hr,IM(+), replicate 1
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
age: 8 weeks
tissue: splenocytes
agent: Imatinib (IM)
Treatment protocol The mouse splenocytes were cultured for 72 h with or without 10 µM Imatinib (IM) .
Extracted molecule total RNA
Extraction protocol QIAGEN RNeasy kit
Label Cy3
Label protocol After fragmentation, 1650 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wash buffer 2 (Agilent)
Hybridization protocol 1650.0 ng at 65 degree_C during 17 hours
Scan protocol Arrays were scanned using Agilent G2565AA Microarray Scanner System.
Description Gene expression in spleen cells cultured with 10 µM Imatinib in conditioning medium containing IL-4 and LPS for 72 h
Data processing Data processed with FeatureExrtraction v9.1. For normalization of raw microarray data, 75 percentile shift normalization was applied with GeneSpring GX v11.2.
Submission date Feb 06, 2012
Last update date Feb 08, 2012
Contact name toyotaka kawamata
Organization name The Institute of Medical Science,The University of Tokyo
Department Hematology-Oncology/Molecular therapy
Street address 4-6-1,Shirokanedai,Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
Platform ID GPL11202
Series (1)
GSE35559 Gene expression profile induced by Imatinib

Data table header descriptions
VALUE Log2 normalized signal intensity

Data table
GE_BrightCorner 3.9013062
DarkCorner -8.070233
A_55_P1989846 -0.46148968
A_55_P1991598 -8.140718
A_55_P2022211 -1.3656411
A_55_P1980764 -4.2666874
A_55_P1964375 1.5548143
A_51_P128876 -2.0986295
A_55_P2121042 -7.423394
A_52_P219230 -7.727904
A_51_P207591 -5.591977
A_55_P2131920 -8.119581
A_55_P2404223 -1.9797878
A_55_P2101944 5.323105
A_52_P358860 -0.033981323
A_51_P119031 -1.142479
A_51_P309854 -8.203393
A_51_P343900 3.7552786
A_51_P234359 -3.5769897
A_51_P487813 1.5669231

Total number of rows: 39485

Table truncated, full table size 923 Kbytes.

Supplementary file Size Download File type/resource
GSM870527.txt.gz 7.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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