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Sample GSM870204 Query DataSets for GSM870204
Status Public on Nov 06, 2012
Title non antibody control ChIPseq
Sample type SRA
 
Source name Fission yeast cells
Organism Schizosaccharomyces pombe
Characteristics strain: 972 h-
develomental stage: No
tap tag: No TAP tag
chip antibody: none
Treatment protocol No
Growth protocol Cells in YEA + 2% glucose were collected in log phase
Extracted molecule genomic DNA
Extraction protocol ChIP assays were performed as described previously [Zhu et al., 2006]. RNA Polymerase II CTD antibody is CTD(4H8) (Abcam, ab5408). Libraries were prepared according to Illumina's instructions.DNA-end was repaired to overhang a 3‟-dA, then adapters were ligated to the end DNA fragments. DNA fragments with proper size (usually 100-300bp, including adaptor sequence) were selected after PCR amplification.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Data processing Bed: Sequence reads were mapped to the ASM294v1 NCBI assembly of the S.pombe genome using Bowtie (version 0.12.7) with default parameters.
Wig: RPM (read per million) counts were calculated from the alignment files and wig files were created with a custom script.
Genome Build:
7-PG.bed: ASM294v1
7-PG.rpm.wig: ASM294v1
 
Submission date Feb 03, 2012
Last update date May 15, 2019
Contact name Claes M Gustafsson
E-mail(s) claes.gustafsson@medkem.gu.se
Organization name Gothenburg University
Department Department of Medical biochemistry and cell biology
Street address Medcinaregatan 9A
City Gothenburg
ZIP/Postal code 405 30
Country Sweden
 
Platform ID GPL13988
Series (2)
GSE35524 Mediator promotes CENP-A incorporation at fission yeast centromeres [ChIP-seq]
GSE35527 Mediator promotes CENP-A incorporation at fission yeast centromeres
Relations
SRA SRX118452
BioSample SAMN00780333

Supplementary file Size Download File type/resource
GSM870204_7-PG.bed.gz 3.4 Mb (ftp)(http) BED
GSM870204_7-PG.rpm.wig.gz 30.0 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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