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Sample GSM8649 Query DataSets for GSM8649
Status Public on Dec 25, 2003
Title Normal Megakaryocytes
Sample type RNA
Source name Bone marrow, TPO treated, CD34 positive cells (6 donors pool)
Organism Homo sapiens
Extracted molecule total RNA
Description Normal donors BM CD34+ cells were differentiated in thrombopoietin-treated liquid suspension cultures. Briefly, CD34+ cells (80,000 /mL) were resuspended in a serum-free medium in the presence of 100 ng/mL of human recombinant thrombopoietin (TPO; Genzyme, Boston, MA). Every 3 days, viable cells were scored by Trypan blue dye exclusion and cultures were amplified with fresh serum-free medium. Each well was then supplemented with 100 ng/mL TPO. After 14 to 16 days of liquid culture, CD34-derived megakaryocytes (MKs) were purified by means of an anti-CD41a monoclonal antibody (MoAb) (Dako, Milan, Italy) directed against the glycoproteic alpha2B-beta3 complex and immunobeads (MPC 450 Dynabeads; Dynal, Oslo, Norway). The purity of MKs was determined for each isolation by indirect immunofluorescence, using an anti-CD41b MoAb which reacts with a different epitope of alpha2B-beta3 subunit (Immunotech Inc., Westbrook, ME), followed by a goat anti-mouse IgG, covalently linked to fluorescein (Becton Dickinson, San Jose, CA).
Submission date Jul 28, 2003
Last update date May 28, 2005
Contact name Enrico Tagliafico
Organization name University of Modena and Reggio Emilia
Department Center for Genome Research
Street address Via Campi 287
City Modena
State/province MO
ZIP/Postal code 41126
Country Italy
Platform ID GPL96
Series (1)
GSE567 Essential Thrombocythemia (ET) vs Normal (baseline) Megakaryocytes

Data table header descriptions
VALUE Affymetrix MAS 5.0 Signal
ABS_CALL Affymetrix MAS 5.0 Detection

Data table
AFFX-BioB-5_at 58.5 P
AFFX-BioB-M_at 107.1 P
AFFX-BioB-3_at 52.4 P
AFFX-BioC-5_at 241.6 P
AFFX-BioC-3_at 183.5 P
AFFX-BioDn-5_at 141.4 P
AFFX-BioDn-3_at 1288.7 P
AFFX-CreX-5_at 1624.5 P
AFFX-CreX-3_at 3144 P
AFFX-DapX-5_at 2.5 A
AFFX-DapX-M_at 9.9 A
AFFX-DapX-3_at 1.6 A
AFFX-LysX-5_at 2.5 A
AFFX-LysX-M_at 11.4 A
AFFX-LysX-3_at 2.8 A
AFFX-PheX-5_at 0.8 A
AFFX-PheX-M_at 4.3 A
AFFX-PheX-3_at 18.1 A
AFFX-ThrX-5_at 1.8 A
AFFX-ThrX-M_at 1.7 A

Total number of rows: 22283

Table truncated, full table size 384 Kbytes.

Supplementary data files not provided

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