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Sample GSM864084 Query DataSets for GSM864084
Status Public on Jan 20, 2012
Title L1236 B_4 D_1 (exon)
Sample type RNA
 
Source name Human Hodgkin Lymphoma Cell Line
Organism Homo sapiens
Characteristics treatment: infected induced shREL-B
cell line: L1236
Treatment protocol HL cell lines were spin-infected using a retrovirus that delivers a specific shRNA to knock down REL-B expression twice in consecutive days. Cell are split into two populations. One is infected and uninduced and the second one is treated with doxyciline to induce the expression of REL-B shRNA.
Growth protocol Hodgkin Lymphoma cells are culture in RPMI medium supplemented with 20% FBS
Extracted molecule total RNA
Extraction protocol TRIZOL
Label biotin
Label protocol Sense strand cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit.
 
Hybridization protocol Samples wre hybridize to Affymetrix human Gene ST 1.0 GeneChIP in Affymetrix hybridization oven at 45C, 60 rpm for 16 hours.
Scan protocol Slides were scanned on Affymetrix geneChip scanner 3000.
Data processing Data were collected using Affymetrix AGCC software. Statistical and clustering analysis was performed with Partek genomics Suite software using RMA normalization algorithm.
 
Submission date Jan 19, 2012
Last update date Jan 20, 2012
Contact name Brian Andrew Lewis
E-mail(s) lewisbri@mail.nih.gov
Phone 301-435-8323
Fax 301-496-9956
Organization name National Cancer Institute-NIH
Department metabolism Branch
Lab Lewis Lab
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10739
Series (1)
GSE35224 Gene expression profiling of Hodgkin Lymphoma cell line following REL-B depletion

Data table header descriptions
ID_REF
VALUE Quantification
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
7892501 7.5692 5.64402e-09
7892502 6.36431 6.80469e-06
7892503 4.56677 0.00498209
7892504 10.0284 3.50094e-05
7892505 3.09309 0.0211524
7892506 6.64007 4.17287e-05
7892507 5.55397 0.0312224
7892508 7.84324 4.72757e-09
7892509 12.0325 4.44414e-09
7892510 8.02056 4.57844e-09
7892511 4.63736 4.99188e-05
7892512 8.13955 0.000187138
7892513 3.49131 0.0575287
7892514 11.115 5.17436e-09
7892515 8.82866 1.02048e-05
7892516 6.92695 1.55688e-07
7892517 7.0812 0.000464799
7892518 4.67659 0.00140109
7892519 6.1313 0.00876064
7892520 9.70821 1.2012e-06

Total number of rows: 257430

Table truncated, full table size 6521 Kbytes.




Supplementary file Size Download File type/resource
GSM864084.CEL.gz 4.1 Mb (ftp)(http) CEL
GSM864084.CHP.gz 2.3 Mb (ftp)(http) CHP
Processed data provided as supplementary file
Processed data included within Sample table

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