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| Status |
Public on Dec 22, 2024 |
| Title |
Kuramochi, BLU 125 nM, rep3 |
| Sample type |
SRA |
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| Source name |
Kuramochi
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| Organism |
Homo sapiens |
| Characteristics |
cell line: Kuramochi
cell type: ovarian cancer
treatment: BLU222, 125 nM
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| Extracted molecule |
total RNA |
| Extraction protocol |
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads
After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
nf-core/rnaseq version 3.3.
Assembly: GRCh38
Supplementary files format and content: Tab delimited text files, contain raw read counts at isoform level.
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| Submission date |
Nov 13, 2024 |
| Last update date |
Dec 22, 2024 |
| Contact name |
Jianxin Wang |
| Organization name |
Roswell Park Comprehensive Cancer Center
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| Department |
Molecular and Cellular Biology
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| Street address |
665 Elm Street
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| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14203 |
| Country |
USA |
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| Platform ID |
GPL11154 |
| Series (1) |
| GSE281746 |
Differential responses to selective CDK2 inhibitor BLU-222 is conditioned by tumor suppressors |
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| Relations |
| BioSample |
SAMN44716895 |
| SRA |
SRX26704697 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM8627471_Kuramochi_BLU_3.isoforms.results.gz |
3.4 Mb |
(ftp)(http) |
RESULTS |
SRA Run Selector |
| Raw data are available in SRA |
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