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Sample GSM85811 Query DataSets for GSM85811
Status Public on Feb 28, 2006
Title Vd2 PBS rep2
Sample type RNA
Source name expanded peripheral blood Vd2 gd T lymphocytes
Organism Homo sapiens
Characteristics healthy female, age 27
Biomaterial provider donor 2
Treatment protocol PBS, 4hr
Growth protocol 3 week culture with Vd1 mab 0.1mg/mL, pan gd mAb 0.1 mg/mL, and IL-15 10ng/mL
Extracted molecule total RNA
Extraction protocol cells were homogenized in TRIzol reagent, then RNA was extracted using a Quagen Rneasy column with no DNase treatment
Label biotin
Label protocol 1mg of total RNA was labelled using the Affymetrix One-Cycle labelling procedure
Hybridization protocol 15mg of labelled RNA was hybridized to the arrays according to standart Affymetrix procedures, then arrays were washed and stained on the Affymetrix GeneChip Fluidics Station 450 using the Midi_euk2v3 protocol
Scan protocol samples were scanned using the Affymetrix GeneChip Scanner 3000
Description expanded Vd2 cells, PBS treated, donor 2
Data processing Affymetrix GCOS
Submission date Dec 02, 2005
Last update date Dec 02, 2005
Contact name Ellen Kress
Phone 406-994-6384
Organization name Montana State University
Department Veterinary Molecular Biology
Lab Jutila
Street address PO Box 173610
City Bozeman
State/province MT
ZIP/Postal code 59717-3610
Country USA
Platform ID GPL571
Series (1)
GSE3720 Distinct gene expression in human Vd1 and Vd2 gd T cells following non-TCR agonist stimulation

Data table header descriptions
ID_REF Affymetrix probe set
VALUE signal intensity
ABS_CALL P for present, M for marginal, A for absent
DETECTION P-VALUE indicates the significance level of the P,A, or M absolute call

Data table
AFFX-BioB-5_at 861.1 P 0.00011
AFFX-BioB-M_at 1433.7 P 0.000044
AFFX-BioB-3_at 883.4 P 0.000044
AFFX-BioC-5_at 1774.2 P 0.00006
AFFX-BioC-3_at 1786.3 P 0.000044
AFFX-BioDn-5_at 2533.7 P 0.000044
AFFX-BioDn-3_at 8203.8 P 0.000044
AFFX-CreX-5_at 16924.1 P 0.000044
AFFX-CreX-3_at 24687.7 P 0.000044
AFFX-DapX-5_at 238.3 P 0.000225
AFFX-DapX-M_at 606.1 P 0.000662
AFFX-DapX-3_at 1053.5 P 0.000052
AFFX-LysX-5_at 50.6 P 0.00762
AFFX-LysX-M_at 79.7 P 0.005565
AFFX-LysX-3_at 170.8 P 0.000972
AFFX-PheX-5_at 57.9 P 0.00034
AFFX-PheX-M_at 98.2 P 0.000857
AFFX-PheX-3_at 101.5 P 0.000662
AFFX-ThrX-5_at 49.7 P 0.002023
AFFX-ThrX-M_at 142.9 P 0.000081

Total number of rows: 22277

Table truncated, full table size 589 Kbytes.

Supplementary data files not provided

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