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Sample GSM85809 Query DataSets for GSM85809
Status Public on Feb 28, 2006
Title Vd1 PMA rep2
Sample type RNA
Source name expanded peripheral blood Vd1 gd T lymphocytes
Organism Homo sapiens
Characteristics healthy female, age 27
Biomaterial provider donor 2
Treatment protocol PMA 20ng/mL, ionomycin 0.5mg/mL, 4hr
Growth protocol 3 week culture with Vd1 mab 0.1mg/mL, pan gd mAb 0.1 mg/mL, and IL-15 10ng/mL
Extracted molecule total RNA
Extraction protocol cells were homogenized in TRIzol reagent, then RNA was extracted using a Quagen Rneasy column with no DNase treatment
Label biotin
Label protocol 1mg of total RNA was labelled using the Affymetrix One-Cycle labelling procedure
Hybridization protocol 15mg of labelled RNA was hybridized to the arrays according to standart Affymetrix procedures, then arrays were washed and stained on the Affymetrix GeneChip Fluidics Station 450 using the Midi_euk2v3 protocol
Scan protocol samples were scanned using the Affymetrix GeneChip Scanner 3000
Description expanded Vd1 cells, PMA treated, donor 2
Data processing Affymetrix GCOS
Submission date Dec 02, 2005
Last update date Dec 02, 2005
Contact name Ellen Kress
Phone 406-994-6384
Organization name Montana State University
Department Veterinary Molecular Biology
Lab Jutila
Street address PO Box 173610
City Bozeman
State/province MT
ZIP/Postal code 59717-3610
Country USA
Platform ID GPL571
Series (1)
GSE3720 Distinct gene expression in human Vd1 and Vd2 gd T cells following non-TCR agonist stimulation

Data table header descriptions
ID_REF Affymetrix probe set
VALUE signal intensity
ABS_CALL P for present, M for marginal, A for absent
DETECTION P-VALUE indicates the significance level of the P,A, or M absolute call

Data table
AFFX-BioB-5_at 1039.5 P 0.000127
AFFX-BioB-M_at 1602 P 0.000044
AFFX-BioB-3_at 972.7 P 0.000044
AFFX-BioC-5_at 1973.1 P 0.000044
AFFX-BioC-3_at 2265 P 0.000044
AFFX-BioDn-5_at 2886.8 P 0.000044
AFFX-BioDn-3_at 9730.4 P 0.000044
AFFX-CreX-5_at 23725.1 P 0.000044
AFFX-CreX-3_at 29276 P 0.000044
AFFX-DapX-5_at 534.6 P 0.000044
AFFX-DapX-M_at 1735.6 P 0.000581
AFFX-DapX-3_at 2129.3 P 0.000052
AFFX-LysX-5_at 90.9 P 0.000195
AFFX-LysX-M_at 153 P 0.013811
AFFX-LysX-3_at 299.2 P 0.000169
AFFX-PheX-5_at 155.6 P 0.000195
AFFX-PheX-M_at 258.8 P 0.000095
AFFX-PheX-3_at 237.8 P 0.000052
AFFX-ThrX-5_at 189.1 P 0.000195
AFFX-ThrX-M_at 291.9 P 0.000044

Total number of rows: 22277

Table truncated, full table size 588 Kbytes.

Supplementary data files not provided

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