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Sample GSM85808 Query DataSets for GSM85808
Status Public on Feb 28, 2006
Title Vd1 PMA rep1
Sample type RNA
Source name expanded peripheral blood Vd1 gd T lymphocytes
Organism Homo sapiens
Characteristics healthy male, age 27
Biomaterial provider donor 1
Treatment protocol PMA 20ng/mL, ionomycin 0.5mg/mL, 4hr
Growth protocol 3 week culture with Vd1 mab 0.1mg/mL, pan gd mAb 0.1 mg/mL, and IL-15 10ng/mL
Extracted molecule total RNA
Extraction protocol cells were homogenized in TRIzol reagent, isopropanol precipitated, DNAse treated, then ethanol precipitated
Label biotin
Label protocol 1mg of total RNA was labelled using the Affymetrix One-Cycle labelling procedure
Hybridization protocol 15mg of labelled RNA was hybridized to the arrays according to standart Affymetrix procedures, then arrays were washed and stained on the Affymetrix GeneChip Fluidics Station 450 using the Midi_euk2v3 protocol
Scan protocol samples were scanned using the Affymetrix GeneChip Scanner 3000
Description expanded Vd1 cells, PMA treated, donor 1
Data processing Affymetrix GCOS
Submission date Dec 02, 2005
Last update date Dec 02, 2005
Contact name Ellen Kress
Phone 406-994-6384
Organization name Montana State University
Department Veterinary Molecular Biology
Lab Jutila
Street address PO Box 173610
City Bozeman
State/province MT
ZIP/Postal code 59717-3610
Country USA
Platform ID GPL571
Series (1)
GSE3720 Distinct gene expression in human Vd1 and Vd2 gd T cells following non-TCR agonist stimulation

Data table header descriptions
ID_REF Affymetrix probe set
VALUE signal intensity
ABS_CALL P for present, M for marginal, A for absent
DETECTION P-VALUE indicates the significance level of the P,A, or M absolute call

Data table
AFFX-BioB-5_at 592.1 P 0.000081
AFFX-BioB-M_at 999.6 P 0.000044
AFFX-BioB-3_at 598.7 P 0.000044
AFFX-BioC-5_at 1597.7 P 0.000044
AFFX-BioC-3_at 2300.3 P 0.000044
AFFX-BioDn-5_at 4158.9 P 0.000044
AFFX-BioDn-3_at 6881.3 P 0.00007
AFFX-CreX-5_at 20335.1 P 0.000052
AFFX-CreX-3_at 21646.9 P 0.000044
AFFX-DapX-5_at 339.9 P 0.00007
AFFX-DapX-M_at 844.7 P 0.000754
AFFX-DapX-3_at 1097.9 P 0.000052
AFFX-LysX-5_at 59.3 P 0.00034
AFFX-LysX-M_at 69.4 P 0.006871
AFFX-LysX-3_at 155.2 P 0.000052
AFFX-PheX-5_at 87.5 P 0.000052
AFFX-PheX-M_at 144.6 P 0.000297
AFFX-PheX-3_at 110.1 P 0.000095
AFFX-ThrX-5_at 89.9 P 0.000581
AFFX-ThrX-M_at 177.8 P 0.000052

Total number of rows: 22277

Table truncated, full table size 587 Kbytes.

Supplementary data files not provided

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