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Sample GSM85807 Query DataSets for GSM85807
Status Public on Feb 28, 2006
Title Vd1 LPS rep2
Sample type RNA
Source name expanded peripheral blood Vd1 gd T lymphocytes
Organism Homo sapiens
Characteristics healthy female, age 27
Biomaterial provider donor 2
Treatment protocol phenol extracted E. coli lipopolysaccharide, 10 mg/mL, 4hr
Growth protocol 3 week culture with Vd1 mab 0.1mg/mL, pan gd mAb 0.1 mg/mL, and IL-15 10ng/mL
Extracted molecule total RNA
Extraction protocol cells were homogenized in TRIzol reagent, then RNA was extracted using a Quagen Rneasy column with no DNase treatment
Label biotin
Label protocol 1mg of total RNA was labelled using the Affymetrix One-Cycle labelling procedure
Hybridization protocol 15mg of labelled RNA was hybridized to the arrays according to standart Affymetrix procedures, then arrays were washed and stained on the Affymetrix GeneChip Fluidics Station 450 using the Midi_euk2v3 protocol
Scan protocol samples were scanned using the Affymetrix GeneChip Scanner 3000
Description expanded Vd1 cells, LPS treated, donor 2
Data processing Affymetrix GCOS
Submission date Dec 02, 2005
Last update date Dec 02, 2005
Contact name Ellen Kress
Phone 406-994-6384
Organization name Montana State University
Department Veterinary Molecular Biology
Lab Jutila
Street address PO Box 173610
City Bozeman
State/province MT
ZIP/Postal code 59717-3610
Country USA
Platform ID GPL571
Series (1)
GSE3720 Distinct gene expression in human Vd1 and Vd2 gd T cells following non-TCR agonist stimulation

Data table header descriptions
ID_REF Affymetrix probe set
VALUE signal intensity
ABS_CALL P for present, M for marginal, A for absent
DETECTION P-VALUE indicates the significance level of the P,A, or M absolute call

Data table
AFFX-BioB-5_at 809.2 P 0.000225
AFFX-BioB-M_at 1412.6 P 0.000044
AFFX-BioB-3_at 735.2 P 0.000052
AFFX-BioC-5_at 1796.4 P 0.000052
AFFX-BioC-3_at 1860.1 P 0.000044
AFFX-BioDn-5_at 2283 P 0.000044
AFFX-BioDn-3_at 7742 P 0.000044
AFFX-CreX-5_at 17920.8 P 0.000044
AFFX-CreX-3_at 25367.7 P 0.000044
AFFX-DapX-5_at 517.4 P 0.000052
AFFX-DapX-M_at 1255.5 P 0.00039
AFFX-DapX-3_at 1832.3 P 0.000044
AFFX-LysX-5_at 55.1 P 0.013811
AFFX-LysX-M_at 138 P 0.004017
AFFX-LysX-3_at 209.2 P 0.000044
AFFX-PheX-5_at 104.5 P 0.000044
AFFX-PheX-M_at 161.3 P 0.000195
AFFX-PheX-3_at 156.4 P 0.000225
AFFX-ThrX-5_at 121.8 P 0.000972
AFFX-ThrX-M_at 243.8 P 0.000052

Total number of rows: 22277

Table truncated, full table size 589 Kbytes.

Supplementary data files not provided

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