phenol extracted E. coli lipopolysaccharide, 10 mg/mL, 4hr
Growth protocol
3 week culture with Vd1 mab 0.1mg/mL, pan gd mAb 0.1 mg/mL, and IL-15 10ng/mL
Extracted molecule
total RNA
Extraction protocol
cells were homogenized in TRIzol reagent, then RNA was extracted using a Quagen Rneasy column with no DNase treatment
Label
biotin
Label protocol
1mg of total RNA was labelled using the Affymetrix One-Cycle labelling procedure
Hybridization protocol
15mg of labelled RNA was hybridized to the arrays according to standart Affymetrix procedures, then arrays were washed and stained on the Affymetrix GeneChip Fluidics Station 450 using the Midi_euk2v3 protocol
Scan protocol
samples were scanned using the Affymetrix GeneChip Scanner 3000
