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Sample GSM85714 Query DataSets for GSM85714
Status Public on Mar 31, 2006
Title bhh treated 3
Sample type RNA
Source name pronephric explant
Organism Xenopus laevis
Characteristics Wild type, Stage 24 pronephric explant
Extracted molecule total RNA
Extraction protocol Pronephric region explants were prepared from stage 24 embryos using a gastromaster (Xenotek) with the tip set to a width of 70 microns. Rectangular explants, approximately 70 microns (dorsal to ventral) x 100 microns (anteriorposterior) and 70 microns (laterally) were cut from the flanks of both sides of embryos. 300 explants were extracted with Trizol (Gibco)
Label biotin
Label protocol 1 µg of total RNA was processed using MessageAmp™ aRNA Amplification Kit (AMBION) to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description St 24 bhh treated pronephric explants
Data processing Affymetrix Microarray Suite version 5.0
Submission date Dec 01, 2005
Last update date Jan 17, 2006
Contact name Curtis R Altmann
Phone 8506442925
Organization name Goodwin Procter LLP
Street address 599 Lexington Avenue
City New York
State/province NY
ZIP/Postal code 10022
Country USA
Platform ID GPL1318
Series (1)
GSE3712 Bhh signaling in pronephric development

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 187.3 P 0.000509
AFFX-BioB-M_at 286.7 P 0.000052
AFFX-BioB-3_at 144.1 P 0.000095
AFFX-BioC-5_at 615.3 P 0.000052
AFFX-BioC-3_at 536.1 P 0.000044
AFFX-BioDn-5_at 973.9 P 0.000044
AFFX-BioDn-3_at 2009.9 P 0.000127
AFFX-CreX-5_at 5606.4 P 0.000044
AFFX-CreX-3_at 7908 P 0.000044
AFFX-DapX-5_at 15.5 A 0.262827
AFFX-DapX-M_at 19.7 A 0.300606
AFFX-DapX-3_at 5.9 A 0.891021
AFFX-LysX-5_at 6.8 A 0.617401
AFFX-LysX-M_at 4.2 A 0.672921
AFFX-LysX-3_at 4.7 A 0.574038
AFFX-PheX-5_at 2.5 A 0.876428
AFFX-PheX-M_at 1.1 A 0.910522
AFFX-PheX-3_at 3.9 A 0.891021
AFFX-ThrX-5_at 16.5 A 0.724854
AFFX-ThrX-M_at 12.9 A 0.455413

Total number of rows: 15611

Table truncated, full table size 504 Kbytes.

Supplementary data files not provided

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