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Sample GSM855196 Query DataSets for GSM855196
Status Public on Dec 31, 2011
Title oocyte from 12day HDAC1 conditional knocked-out mouse, biological rep3
Sample type RNA
 
Source name oocyte from 12day HDAC1 conditional knocked-out
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: HDAC1 conditional knocked-out
age: 12day-old
tissue source: ovary
tissue: oocyte
Treatment protocol To establish the in vivo role(s) of HDAC1 and HDAC2 during oocyte growth, we generated conditional mutant oocytes using Zp3-Cre transgenic mice (PMID 9016703, 12912906) in combination with conditional loss of function alleles of Hdac1 or/and Hdac2
Growth protocol from 12 day old mouse ovary
Extracted molecule total RNA
Extraction protocol using the PicoPure RNA kit (Arcturus) according to the manufacturer's instructions.
Label biotin
Label protocol FL-OvationTM cDNA Biotin Module V2 (NuGen)
 
Hybridization protocol Following fragmentation, Four independent biological replicates were hybridized to Affymetrix GeneChip Mouse 1.1 ST microarrays
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from oocyte from 12day mouse
Data processing Raw microarray data were analyzed as previously described using MAS5, GeneSpring v7, SAM and EASE software
 
Submission date Dec 29, 2011
Last update date Jan 02, 2012
Contact name Hua Pan
E-mail(s) huapan@sas.upenn.edu
Organization name University of Pennsylvania
Department Department of Biology
Street address 415 S University Avenue
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL11533
Series (1)
GSE34786 Expression data from HDAC1 and HDAC2 knocked-out mouse oocyte

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
10338001 11.18674
10338002 3.721277
10338003 9.198015
10338004 8.815348
10338005 2.680171
10338006 2.807302
10338007 2.862532
10338008 3.013372
10338009 5.47166
10338010 2.689704
10338011 3.451046
10338012 2.723804
10338013 2.565387
10338014 2.607488
10338015 2.541911
10338016 4.404568
10338017 12.37287
10338018 3.951645
10338019 3.30731
10338020 5.634785

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM855196_HD1_rep3.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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