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Status |
Public on Dec 31, 2011 |
Title |
oocyte from 12day HDAC1 conditional knocked-out mouse, biological rep3 |
Sample type |
RNA |
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|
Source name |
oocyte from 12day HDAC1 conditional knocked-out
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Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 genotype/variation: HDAC1 conditional knocked-out age: 12day-old tissue source: ovary tissue: oocyte
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Treatment protocol |
To establish the in vivo role(s) of HDAC1 and HDAC2 during oocyte growth, we generated conditional mutant oocytes using Zp3-Cre transgenic mice (PMID 9016703, 12912906) in combination with conditional loss of function alleles of Hdac1 or/and Hdac2
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Growth protocol |
from 12 day old mouse ovary
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Extracted molecule |
total RNA |
Extraction protocol |
using the PicoPure RNA kit (Arcturus) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
FL-OvationTM cDNA Biotin Module V2 (NuGen)
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Hybridization protocol |
Following fragmentation, Four independent biological replicates were hybridized to Affymetrix GeneChip Mouse 1.1 ST microarrays
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Description |
Gene expression data from oocyte from 12day mouse
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Data processing |
Raw microarray data were analyzed as previously described using MAS5, GeneSpring v7, SAM and EASE software
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Submission date |
Dec 29, 2011 |
Last update date |
Jan 02, 2012 |
Contact name |
Hua Pan |
E-mail(s) |
huapan@sas.upenn.edu
|
Organization name |
University of Pennsylvania
|
Department |
Department of Biology
|
Street address |
415 S University Avenue
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
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|
Platform ID |
GPL11533 |
Series (1) |
GSE34786 |
Expression data from HDAC1 and HDAC2 knocked-out mouse oocyte |
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