|
Status |
Public on Dec 22, 2011 |
Title |
NA_CON_1Y_1 |
Sample type |
genomic |
|
|
Source name |
gDNA from CD4+ cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: 12m CD4+ gender: female age: 1 disease state: NORM treatment: CON pool id: D
|
Treatment protocol |
Anti-CD3 (0.5ug/mL) + rhIL2 (20Units) or UNTREATED
|
Growth protocol |
cells cultured for 24 hours in AIM-V media + 2Mercapto ethanol (serum free)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA and RNA co purified from source according to Qiagen all prep kit protocol
|
Label |
Cy5 and Cy3
|
Label protocol |
Cy5 and Cy3
|
|
|
Hybridization protocol |
Infinium Methylation Assay according to manufacturers protocol (Illumina)
|
Scan protocol |
iScan system
|
Description |
12m CD4+
|
Data processing |
Genome studio v2010.3 with Methylation Module v1.8.5 under default settings
|
|
|
Submission date |
Dec 21, 2011 |
Last update date |
Dec 22, 2011 |
Contact name |
David Martino |
E-mail(s) |
david.martino@telethonkids.org.au
|
Phone |
0863191635
|
Organization name |
Telethon Kids Institute
|
Street address |
Perth Childrens Hospital, 15 Hospital Avenue
|
City |
NEDLANDS |
State/province |
Western Australia |
ZIP/Postal code |
6009 |
Country |
Australia |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE34639 |
Methylation data from CD4+ cells at birth and 12 months from a cohort of children who were diagnosed with IgE food allergy to either egg milk or peanut at 12 months (n=12) and healthy controls (n=12). |
|