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Sample GSM851665 Query DataSets for GSM851665
Status Public on Feb 21, 2012
Title IgG control rep2
Sample type SRA
 
Source name adult testis
Organism Mus musculus
Characteristics age: adult
gender: male
genetic background: 13R female x 9R male F1
genotype: Hop2 -/-
chip antibody: IgG
Growth protocol Cells were extracted from testis of euthanized mice and subjected to immunoprecipitation.
Extracted molecule genomic DNA
Extraction protocol Lysates from mouse testis cells were crosslinked, sonicated and immunoprecipitated with anti-DMC1 or anti-RAD51 antibodies. Library construction was done using enzymes from New England Biolabs or Illumina and standard Illumina protocol. Size selection of library fragments of 150~250 bp was performed after PCR amplification. “Klenow exo(-)” sample was prepared by substituting Klenow fragment and T4 DNA polymerase from the end repair step of the standard protocol with Klenow exo(-) enzyme and performing end repair at 37 °C for 1h. “Klenow exo(-), 12 °C“ sample was prepared the same way followed by incubation at 20 °C for 2h and at 12 °C overnight. "Kinetic enrichment" samples were denatured and quickly renatured before adapter ligation.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
 
Description Standard ChIP-Seq.
Data processing IgG2.sorted.ssDNA_type1.bed; genome build: mm9
IgG2.sorted.ssDNA_type2.bed; genome build: mm9
IgG2.sorted.dsDNA.bed; genome build: mm9
IgG2.sorted.1K_100.wig; genome build: mm9
BED: Quality-filtered sequencing reads were mapped to mm9 version of mouse genome assembly using BWA (first end) (http://bio-bwa.sourceforge.net/) or BWA-RA (second end). BWA-RA is modification of BWA that iteratevily seeks longest aligning suffux of the query. Obtained alignments were further processed to generate ssDNA type 1, ssDNA type 2 and dsDNA subsets of fragments. Fourths field in the BED file is (ITR_length)_(microhomology_lenght) and fifth field is BWA-calculated alignment quality. PHRED alignment qualities for both ends (0-30) were added and then re-scaled to 0-1000.
WIG: type1 ssDNA, type2 ssDNA and dsDNA subsets were combined and read coverage was calculated in sliding windows of 1Kb with 100 bp step. Counts were normalized to total numer of reads in the dataset and are given in reads per million.
 
Submission date Dec 20, 2011
Last update date May 15, 2019
Contact name Pavel P Khil
E-mail(s) pk94j@nih.gov
Phone 301-496-4638
Fax (301)-496-9878
Organization name NIDDK
Department
Lab Genetics and Biochemistry Branch
Street address 5 Memorial Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL9250
Series (1)
GSE34592 Sequencing-based ssDNA detection
Relations
SRA SRX112535
BioSample SAMN00767718

Supplementary file Size Download File type/resource
GSM851665_IgG2.sorted.1K_100.wig.gz 183.6 Mb (ftp)(http) WIG
GSM851665_IgG2.sorted.dsDNA.bed.gz 55.4 Mb (ftp)(http) BED
GSM851665_IgG2.sorted.ssDNA_type1.bed.gz 1.5 Mb (ftp)(http) BED
GSM851665_IgG2.sorted.ssDNA_type2.bed.gz 610.4 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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