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Sample GSM850908 Query DataSets for GSM850908
Status Public on Jul 01, 2012
Title RenLab-RNA-Seq-E14.5-limb
Sample type SRA
Source name Mouse E14.5 limb
Organism Mus musculus
Characteristics strain: C57Bl/6
Treatment protocol None
Growth protocol Adult intestine, olfactory bulb, testis, and thymus were dissected from 8-week old male C57Bl/6 mice (Charles River). Placenta was extracted from C57Bl/6 pregnant mice. E14.5 brain, heart, limb and liver were derived from E14.5 C57Bl/6 mouse embryos.
Extracted molecule polyA RNA
Extraction protocol RNA samples from tissues and primary cells were extracted from Trizol according to protocol (Invitrogen). polyA+RNA was purified with the Dynabeads mRNA purification kit (Invitrogen). The mRNA libraries were prepared for strand-specific sequencing as described previously in Parkhomchuk, D. et al. (2009).
Library strategy RNA-Seq
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 2000
Data processing fastq: Illumina's HiSeq Control Software
bam and bigWig: Alignment to mm9 using TopHat with default parameters.
Submission date Dec 19, 2011
Last update date May 15, 2019
Contact name Bing Ren
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platform ID GPL13112
Series (2)
GSE29184 A draft map of cis-regulatory sequences in the mouse genome
GSE29278 A draft map of cis-regulatory sequences in the mouse genome [RNA-Seq]
SRA SRX113071
BioSample SAMN00768229
Named Annotation GSM850908_limb-E14.5-1.bigwig
Named Annotation GSM850908_limb-E14.5-2.bigwig

Supplementary file Size Download File type/resource
GSM850908_limb-E14.5-1.bam 753.6 Mb (ftp)(http) BAM
GSM850908_limb-E14.5-1.bigwig 75.7 Mb (ftp)(http) BIGWIG
GSM850908_limb-E14.5-2.bam 719.0 Mb (ftp)(http) BAM
GSM850908_limb-E14.5-2.bigwig 72.6 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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