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Sample GSM850906 Query DataSets for GSM850906
Status Public on Jul 01, 2012
Title RenLab-RNA-Seq-E14.5-brain
Sample type SRA
 
Source name Mouse E14.5 brain
Organism Mus musculus
Characteristics strain: C57Bl/6
Treatment protocol None
Growth protocol Adult intestine, olfactory bulb, testis, and thymus were dissected from 8-week old male C57Bl/6 mice (Charles River). Placenta was extracted from C57Bl/6 pregnant mice. E14.5 brain, heart, limb and liver were derived from E14.5 C57Bl/6 mouse embryos.
Extracted molecule polyA RNA
Extraction protocol RNA samples from tissues and primary cells were extracted from Trizol according to protocol (Invitrogen). polyA+RNA was purified with the Dynabeads mRNA purification kit (Invitrogen). The mRNA libraries were prepared for strand-specific sequencing as described previously in Parkhomchuk, D. et al. (2009).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 2000
 
Data processing fastq: Illumina's HiSeq Control Software
bam and bigWig: Alignment to mm9 using TopHat with default parameters.
 
Submission date Dec 19, 2011
Last update date May 15, 2019
Contact name Bing Ren
E-mail(s) biren@ucsd.edu
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platform ID GPL13112
Series (2)
GSE29184 A draft map of cis-regulatory sequences in the mouse genome
GSE29278 A draft map of cis-regulatory sequences in the mouse genome [RNA-Seq]
Relations
SRA SRX113069
BioSample SAMN00768227
Named Annotation GSM850906_brain-E14.5-1.bigwig
Named Annotation GSM850906_brain-E14.5-2.bigwig

Supplementary file Size Download File type/resource
GSM850906_brain-E14.5-1.bam 777.2 Mb (ftp)(http) BAM
GSM850906_brain-E14.5-1.bigwig 71.7 Mb (ftp)(http) BIGWIG
GSM850906_brain-E14.5-2.bam 713.1 Mb (ftp)(http) BAM
GSM850906_brain-E14.5-2.bigwig 75.0 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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