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Sample GSM849985 Query DataSets for GSM849985
Status Public on Apr 10, 2012
Title HDLEC_3307.L
Sample type genomic
Source name Dermal lymphatic endothelial cells_buttock
Organism Homo sapiens
Characteristics gender: female
age: 70 years
tissue: buttock
cell type: dermal lymphatic endothelial cells
Growth protocol Endothelial cells were grown in EGM-2 MV contained Endothelial Cell Basal Medium-2 (EBM-2) supplemented with 25 ml FBS, 0.2 ml hydrocortisone, 2 ml hFGF, 0.5 ml VEGF, 0.5 ml R3-IGF-1, 0.5
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted and purified from skin biopsy samples using QIAamp DNA Investigator Kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol bisulphite conversion and subsequent cleanup was carried out using EZ DNA Methylation™ Kit (D5001), Zymo Research; converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using the standard Illumina protocol
Scan protocol Arrays were imaged using iScan Reader using standard recommended Illumina scanner setting
Description punch biopsy
Data processing BeadStudio software 2011.1 and R statistical software vers. 2.12.1 Non_normalized data matrix contains unmethylated (signal A) and methylated (signal B) signal intensities.
Submission date Dec 16, 2011
Last update date Apr 10, 2012
Contact name Silvia Rueberg
Organization name Miltenyi Biotec GmbH
Department Genomic Services Department
Street address Friedrich-Ebert-Str. 68
City Bergisch Gladbach
ZIP/Postal code 51429
Country Germany
Platform ID GPL13534
Series (2)
GSE34486 DNA methylation regulates lineage-specifying genes in the human vascular system [methylation array].
GSE34487 DNA methylation regulates lineage-specifying genes in the human vascular system.

Data table header descriptions
VALUE Average Beta, quantile-normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
cg00000029 0.419279983 0
cg00000108 0.91181089 0
cg00000109 0.818595136 0
cg00000165 0.20192014 0
cg00000236 0.611021103 0
cg00000289 0.504321206 0
cg00000292 0.695339613 0
cg00000321 0.272418664 0
cg00000363 0.174444972 0
cg00000622 0.020334177 0
cg00000658 0.812259149 0
cg00000714 0.205764933 0
cg00000721 0.857114779 0
cg00000734 0.099811102 0
cg00000769 0.025235316 0
cg00000807 0.484086591 0
cg00000884 0.843791642 0
cg00000905 0.108509105 0
cg00000924 0.434037882 0
cg00000948 0.861931521 0

Total number of rows: 485512

Table truncated, full table size 11815 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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