|
| Status |
Public on Sep 04, 2024 |
| Title |
Control muscle |
| Sample type |
SRA |
| |
|
| Source name |
skeletal muscle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skeletal muscle
cell line: none
cell type: none
genotype: wild-type
treatment: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). Total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using NEBNext Multiplex Small RNA Library Prep kit (New England BioLabs, Inc., USA) according to the manufacturer’ instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
skeletal muscle without exercise
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using trimmomatic-0.39 and cutadapt 2.8), then mapped to mm10 whole genome using STAR-2.5.3a
The quantification of reads was processed using COMPSRA.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include read count values for each Sample
|
| |
|
| Submission date |
Sep 04, 2024 |
| Last update date |
Sep 05, 2024 |
| Contact name |
Eui-Ju Hong |
| E-mail(s) |
euijuhong@gmail.com
|
| Phone |
01082555483
|
| Organization name |
Chungnam National University (CNU)
|
| Street address |
99 Dahak-ro, #412, Veterinary Building, Daejeon, 34134
|
| City |
Daejeon |
| State/province |
Not Applicable |
| ZIP/Postal code |
34134 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL21626 |
| Series (1) |
| GSE276372 |
Exercise promotes peripheral glycolysis through miR-204 induction in skeletal muscle |
|
| Relations |
| BioSample |
SAMN43500017 |
| SRA |
SRX25971693 |
